Restorative Dentistry & Endodontics

Research Article

The relationship between the level of salivary alpha amylase activity and pain severity in patients with symptomatic irreversible pulpitis: Fatemeh Ahmadi-Motamayel, Shahriar Shahriari, Mohammad Taghi Goodarzi, Abbas Moghimbeigi, Mina Jazaeri, Parisa Babaei; Restor Dent Endod 2013;38(3):141-145. Published online August 23, 2013; DOI: https://doi.org/10.5395/rde.2013.38.3.141

Objectives

Assessment of dental pain severity is very challenging in dentistry. Previous studies have suggested that elevated salivary alpha amylase may contribute to increased physical stresses. There is a close association between salivary alpha amylase and plasma norepinephrine under stressful physical conditions. The aim of this study was to evaluate the relationship between pain severity and salivary alpha amylase levels in patients with symptomatic irreversible pulpitis.

Materials and Methods

Thirty-six patients (20 females and 16 males) with severe tooth pain due to symptomatic irreversible pulpitis were selected. The visual analogue scale (VAS) score was used to assess the pain severity in each patient. Unstimulated whole saliva was collected, and the level of alpha amylase activity was assessed by the spectrophotometric method. Statistical analysis was performed using SPSS 13.

Results

The level of alpha amylase was significantly increased in the saliva in association with pain severity assessed by VAS. The salivary alpha amylase was also elevated with increased age and in males.

Conclusions

There was a significant correlation between the VAS pain scale and salivary alpha amylase level, which indicates this biomarker may be a good index for the objective assessment of pain intensity.

Citations

Citations to this article as recorded by

Diagnosis of Pain Deception Using Minnesota Multiphasic Personality Inventory-2 Based on XGBoost Machine Learning Algorithm: A Single-Blinded Randomized Controlled Trial
Hyewon Chung, Kihwan Nam, Subin Lee, Ami Woo, Joongbaek Kim, Eunhye Park, Hosik Moon
Medicina.2024; 60(12): 1989. CrossRef
Utilizing Saliva Biomarkers for Diagnostic Purposes
Fatemeh Ahmadi -Motamayel, Ali Mahdavinezhad, Seyedeh Sareh Hendi
Avicenna Journal of Dental Research.2024; 16(1): 46. CrossRef
Clinical correlation of salivary alpha-amylase levels with pain intensity in patients undergoing emergency endodontic treatment
Kavalipurapu Venkata Teja, Sindhu Ramesh, Krishnamachari Janani, Kumar Chandan Srivastava, Deepti Shrivastava, Valentino Natoli, Marco Di Blasio, Marco Cicciù, Giuseppe Minervini
BMC Oral Health.2023;[Epub] CrossRef
Statherin and alpha-amylase levels in saliva from patients with gingivitis and periodontitis
Hanife Merva Parlak, Esra Buber, Ali Tugrul Gur, Erdem Karabulut, Ferda Alev Akalin
Archives of Oral Biology.2023; 145: 105574. CrossRef
Topical review – salivary biomarkers in chronic muscle pain
Hajer Jasim
Scandinavian Journal of Pain.2023; 23(1): 3. CrossRef
Relationship between salivary alpha-amylase activity and pain relief scale score after low level laser therapy: a prospective interventional pilot study
Ryoji Iida, Junpei Konishi, Takahiro Suzuki
Lasers in Medical Science.2022; 37(1): 681. CrossRef
Assessment of salivary stress and pain biomarkers and their relation to self-reported pain intensity during orthodontic tooth movement: a longitudinal and prospective study
Nehir Canigur Bavbek, Erdal Bozkaya, Sila Cagri Isler, Sehri Elbeg, Ahu Uraz, Sema Yuksel
Journal of Orofacial Orthopedics / Fortschritte der Kieferorthopädie.2022; 83(5): 339. CrossRef
Pain intensity and salivary α‐amylase activity in patients following mandibular third molar surgery
Wanvipa Surin, Piyanart Chatiketu, Nuntouchaporn Hutachok, Somdet Srichairatanakool, Vuttinun Chatupos
Clinical and Experimental Dental Research.2022; 8(5): 1082. CrossRef
Application of Salivary Biomarkers in the Diagnosis of Fibromyalgia
Rebeca Illescas-Montes, Víctor J. Costela-Ruiz, Lucía Melguizo-Rodríguez, Elvira De Luna-Bertos, Concepción Ruiz, Javier Ramos-Torrecillas
Diagnostics.2021; 11(1): 63. CrossRef
The Correlation between Pain, Stress, and Oral Function in Oral and Maxillofacial Infection and Trauma Patients
Medyannisa Shafira, Tantry Maulina, Nurnayly Putri Lyana, Endang Sjamsudin, Andri Hardianto
The Open Dentistry Journal.2021; 15(1): 266. CrossRef
Comparison of the Zurich Observation Pain Assessment with the Behavioural Pain Scale and the Critical Care Pain Observation Tool in nonverbal patients in the intensive care unit: A prospective observational study
Martin R. Fröhlich, Gabriele Meyer, Rebecca Spirig, Lucas M. Bachmann
Intensive and Critical Care Nursing.2020; 60: 102874. CrossRef
Salivary Alpha-Amylase in Experimentally-Induced Muscle Pain
Nikolaos Christidis, Pegah Baghernejad, Aylin Deyhim, Hajer Jasim
Diagnostics.2020; 10(9): 722. CrossRef
Protein Signature in Saliva of Temporomandibular Disorders Myalgia
Hajer Jasim, Malin Ernberg, Anders Carlsson, Björn Gerdle, Bijar Ghafouri
International Journal of Molecular Sciences.2020; 21(7): 2569. CrossRef
The Relationship Between Salivary Alpha Amylase Activity and Score of McGill Pain Questionnaire in Patients With Tension Type Headache
Mohammad Vahedi, Mehrdokht Mazdeh, Mehrdad Hajilooi, Maryam Farhadian, Yasamin Barakian, Parastoo Sadr
Basic and Clinical Neuroscience Journal.2018; 9(1): 59. CrossRef
Salivary Stress-Related Responses in Tinnitus: A Preliminary Study in Young Male Subjects with Tinnitus
Ola A. Alsalman, Denise Tucker, Sven Vanneste
Frontiers in Neuroscience.2016;[Epub] CrossRef
Pain during embryo transfer is independently associated with clinical pregnancy in fresh/frozen assisted reproductive technology cycles
Sotirios H. Saravelos, Alice WY. Wong, Grace WS. Kong, Jin Huang, Robert Klitzman, Tin‐Chiu Li
Journal of Obstetrics and Gynaecology Research.2016; 42(6): 684. CrossRef

435 View
7 Download
16 Crossref

Basic Researchs

The effect of tumor necrosis factor (TNF)-α to induce matrix metalloproteinase (MMPs) from the human dental pulp, gingival, and periodontal ligament cells: Eun-Mi Rhim, Sang-Hyuk Park, Duck-Su Kim, Sun-Young Kim, Kyoung-Kyu Choi, Gi-Woon Choi; J Korean Acad Conserv Dent 2011;36(1):26-36. Published online January 31, 2011; DOI: https://doi.org/10.5395/JKACD.2011.36.1.26

Abstract PDF PubReader ePub

Objectives

In the present study, three kinds of tissues cells (pulp, gingiva, and periodontal ligament) were investigated if those cells express MMP and TIMP when they were stimulated with neuropeptides (substance P, CGRP) or proinflammatory cytokine, TNF-α.

Materials and Methods

The cells cultured from human dental pulp (PF), gingiva (GF) and periodontal ligament were (PDLF) stimulated with Mock, SP, TNF-α, and CGRP for 24 hrs and 48 hrs. for an RNase protection assay and Enzyme Linked Immunosorbent Assay.

Cells (PF, GF and PDLF) seeded in 100 mm culture dish were stimulated with SP (10^-5, 10^-8 M) or only with medium (Mock stimulation) for 4hrs and for 24 hrs for RNase Protection Assay, and they were stimulated with CGRP (10^-5 M) and TNF-α (2 ng/mL) for 24 hrs and with various concentraion of TNF-α (2, 10, and 100 ng/mL) for Rnase Protection Assay with a human MMP-1 probe set including MMP 1, 2, 8, 7, 8, 9, 12, and TIMP 2, 3.

In addition, cells (PF, GF and PDLF) were stimulated with Mock and various concentraion of TNF-α (2, 10, and 100 ng/mL) for 24 hrs and with TNF-α (10 ng/mL) for 48 hrs, and the supernatents from the cells were collected for Enzyme Linked Immunosorbent Assay (ELISA) for MMP-1 and MMP-13.

Results

The expression of MMPs in PF, GF, PDLF after stimulation with SP and CGRP were not changed compared with Mock stimulation for 4 hrs and 24 hrs. The expression of MMP-1, -12, -13 24 hrs after stimulation with TNF-α were upregulated, however the expression of TIMP-3 in PF, GF, PDLF after stimulation with TNF-α were downregulated. TNF-α (2 ng/mL, 10 ng/mL, 100 ng/mL) increased MMP-1 and MMP-12 expression in PF dose dependently for 24 hrs.

Conclusions

TNF-α in the area of inflammation may play an important role in regulating the remodeling of dentin, cementum, and alveolar bone.

Citations

Citations to this article as recorded by

Anti‐Inflammatory Effects of Melatonin and 5‐Methoxytryptophol on Lipopolysaccharide‐Induced Acute Pulpitis in Rats
Fatma Kermeoğlu, Umut Aksoy, Abdullah Sebai, Gökçe Savtekin, Hanife Özkayalar, Serkan Sayıner, Ahmet Özer Şehirli, Shuai CHEN
BioMed Research International.2021;[Epub] CrossRef
Cross-Talk between Ciliary Epithelium and Trabecular Meshwork Cells In-Vitro: A New Insight into Glaucoma
Natalie Lerner, Elie Beit-Yannai, Wayne Iwan Lee Davies
PLoS ONE.2014; 9(11): e112259. CrossRef

283 View
2 Download
2 Crossref

The effect of several root-end filling materials on MG63 osteoblast-like cells: Jeong-Ho Lee, Won-Jun Shon, WooCheol Lee, Seung-Ho Baek; J Korean Acad Conserv Dent 2010;35(3):222-228. Published online May 31, 2010; DOI: https://doi.org/10.5395/JKACD.2010.35.3.222

Abstract PDF PubReader ePub

The purpose of this study was to compare mineral trioxide aggregate (MTA; Dentsply, Tulsa Dental, Tulsa, OK, USA), which is widely used as root-end filling material, with DiaRoot BioAggregate (DB; Innovative BioCaramix Inc, Vancouver, BC, Canada), newly developed product, by using MG63 osteoblast-like cells. MTA, DB, and Intermediate Restorative Material (IRM; Dentsply Caulk, Milford, DE, USA) were used for root-end filling material while tissue culture plastic was used for control group. Each material was mixed and, the mixtures were left to set for 24 hours. MG63 cells were seeded to each group and then they were cultured for attachment for 4 hours. Following the attachment of cells to the root-end filling material, early cellular response was observed. After another 12 hours'culture, the level of attachment between cells and material was observed and in order to identify the effect of each material to bone formation, transforming growth factor beta1 (TGFβ1) and osteocalin (OC) were estimated by using enzyme-linked immunosorbent assay (ELISA), and the amount of alkaline phosphatase (ALP) was also measured. The data were analyzed using one-way ANOVA. As a result, only at OC and the number of cells which were attached to materials, there was no statistical difference between MTA and DB. At other items, there was statistically significant difference in all groups. Although DB has not shown exactly the same cellular response like that of MTA, the number of attached cells shows that biocompatibility of the material and OC indicates bone formation rate. Therefore, if DB is used for root end filling material, it is expected to lead to similar results to MTA.

Citations

Citations to this article as recorded by

Comparative analysis of physicochemical properties of root perforation sealer materials
Maura Cristiane Gonçales Orçati Dorileo, Fábio Luis Miranda Pedro, Matheus Coelho Bandeca, Orlando Aguirre Guedes, Ricardo Dalla Villa, Alvaro Henrique Borges
Restorative Dentistry & Endodontics.2014; 39(3): 201. CrossRef
Biocompatibility of root-end filling materials: recent update
Payal Saxena, Saurabh Kumar Gupta, Vilas Newaskar
Restorative Dentistry & Endodontics.2013; 38(3): 119. CrossRef

293 View
1 Download
2 Crossref

Original Article

A bioactivity study of Portland cement mixed with β-glycerophosphosphate on human pulp cell: Young-Hwan Oh, Young-Joo Jang, Yong-Bum Cho; J Korean Acad Conserv Dent 2009;34(5):415-423. Published online September 30, 2009; DOI: https://doi.org/10.5395/JKACD.2009.34.5.415

Abstract PDF PubReader ePub

The purpose of this study is to investigate the response of human pulp cell on Portland cement mixed with β-glycerophosphate. To investigate the effect of β-glycerophosphate and/or dexamethasone on human pulp cell, ALP activity on various concentration of β-glycerophosphate and dexamethasone was measured and mineral nodule of human pulp cell was stained with Alizarin red S. MTS assay and ALP activity of human pulp cell on Portland cement mixed with various concentration of β-glycerophosphate (10 mM, 100mM, 1M) was measured and the specimens were examined under SEM.

Addition of β-glycerophosphate or dexamethasone alone had no effect however, the addition of 5 mM β-glycerophosphate and 100 nM dexamethasone had the largest increasement in ALP activity. There was no toxicity in all samples and the data showed that Portland cement mixed with 10 mM β-glycerophosphate had more increase in ALP activity compared with control.

In conclusion, Portland cement mixed with β-glycerophosphate has no toxicity and promotes differentiation and mineralization of pulp cell compared with additive-free Portland cement. This implicated that application of Portland cement mixed with β-glycerophosphate might form more reparative dentin and in turn it would bring direct pulp capping to success.

Citations

Citations to this article as recorded by

Dentinogenic potential of human adult dental pulp cells during the extended primary culture
Jin-Hee Min, Seon-Yle Ko, Yong-Bum Cho, Chun-Jeih Ryu, Young-Joo Jang
Human Cell.2011; 24(1): 43. CrossRef

269 View
1 Download
1 Crossref

First
Prev
Page of 1
Next
Last

Search