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Research Article
Effect of three nanobiomaterials on microhardness of bleached enamel
Maryam Khoroushi, Farinaz Shirban, Sara Kaveh, Samaneh Doustfateme
Restor Dent Endod 2016;41(3):196-201.   Published online July 14, 2016
DOI: https://doi.org/10.5395/rde.2016.41.3.196
AbstractAbstract PDFPubReaderePub
Objectives

The aim of this in vitro study was to evaluate the effect of incorporating three different nanobiomaterials into bleaching material on microhardness of bleached enamel.

Materials and Methods

The crowns of 24 extracted sound human molars were sectioned. Sixty enamel specimens (2 × 3 × 4 mm) were selected and divided into five groups (n = 12): Group 1 received no bleaching procedure (control); Group 2 underwent bleaching with a 40% hydrogen peroxide (HP) gel; Groups 3, 4, and 5 were bleached with a 40% HP gel modified by incorporation of bioactive glass (BAG), amorphous calcium phosphate (ACP) and hydroxyapatite (HA), respectively. The enamel microhardness was evaluated. The differences in Knoop microhardness data of each group were analyzed by one-way ANOVA, followed by post hoc Tukey tests.

Results

Significant differences were observed between the study groups. The enamel microhardness changes in Groups 1, 3, 4, and 5 were significantly lower than that of Group 2 (p < 0.001).

Conclusions

Within the limitations of this study, it can be concluded that incorporation of each one of the three tested biomaterials as remineralizing agents might be effective in decreasing enamel microhardness changes subsequent to in-office bleaching.

Citations

Citations to this article as recorded by  
  • Protective role of calcium-based agents in dental bleaching gels: insights from a systematic review and meta-analysis of clinical and laboratory evidence
    Gabriel Pereira Nunes, Renata de Oliveira Alves, Geórgia Rondó Peres, Matheus Henrique Faccioli Ragghianti, Priscila Toninatto Alves de Toledo, Alexandre Henrique dos Reis Prado, Carla Ferreira-Baptista, Alberto Carlos Botazzo Delbem
    Clinical Oral Investigations.2025;[Epub]     CrossRef
  • Analysis of Enamel Surface and Shear Bond Strength with Orthodontic Bracket at Different Time Intervals After Bleaching Treatment
    Yu-Jin Lee, Ji-Yeon Hong, Hye-Min Ku, Song-Yi Yang
    Journal of Dental Hygiene Science.2025; 25(2): 130.     CrossRef
  • Effect of strontium fluorophosphate bioactive glass on color, microhardness and surface roughness of bleached enamel
    Shiza Yezdani, Monisha Khatri, Sampath Vidhya, Sekar Mahalaxmi
    Technology and Health Care.2024; 32(1): 285.     CrossRef
  • In vitro evaluation of the effect of addition of biomaterials to carbamide peroxide on the bleaching efficacy and microhardness of enamel
    Sowmya Kavoor, M. A. Ranjini, Naval Abdul Aziz, H. K. Ashok, Roopa R. Nadig
    Journal of Conservative Dentistry and Endodontics.2024; 27(3): 310.     CrossRef
  • Effect of hydrogen peroxide and its combination with nano-hydroxyapatite or nano-bioactive glass on the enamel demineralization and tooth color: An in vitro study
    Elham Kheradmand, Alirea Daneshkazemi, Abdolrahim Davari, Maede Kave, Solmaz Ghanbarnejad
    Dental Research Journal.2023;[Epub]     CrossRef
  • Over‐the‐counter bleaching agents can help with tooth whitening maintenance
    Olívia Santana Jorge, Carolina Noronha Ferraz de Arruda, Rafaella Tonani Torrieri, Rocio Geng Vivanco, Fernanda de Carvalho Panzeri Pires‐de‐Souza
    Journal of Esthetic and Restorative Dentistry.2022; 34(2): 328.     CrossRef
  • Effect of Indigenously Developed Nano-Hydroxyapatite Crystals from Chicken Egg Shell on the Surface Hardness of Bleached Human Enamel
    Divya Kunam, Vidhya Sampath, Sujatha Manimaran, Mahalaxmi Sekar
    Contemporary Clinical Dentistry.2019; 10(3): 489.     CrossRef
  • Influence of Time Intervals between Bleaching Procedures on Enamel Microhardness and Surface Roughness
    Roberta Pimentel de Oliveira, Juliana Costa Pereira Baia, Mara Eliane Soares Ribeiro, Mario Honorato da Silva e Souza Junior, Sandro Cordeiro Loretto
    The Open Dentistry Journal.2018; 12(1): 555.     CrossRef
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Original Article
Cytotoxicity and genotoxicity of newly developed calcium phosphate-based root canal sealers
Hee-Jung Kim, Seung-Ho Baek, Kwang-Shik Bae
J Korean Acad Conserv Dent 2006;31(1):36-49.   Published online January 31, 2006
DOI: https://doi.org/10.5395/JKACD.2006.31.1.036
AbstractAbstract PDFPubReaderePub

The purpose of this study was to compare the cytotoxicity by MTT test and genotoxicity by Ames test of new calcium phosphate-based root canal sealers (CAPSEAL I, CAPSEAL II) with commercially available resin-based sealers (AH 26, AH Plus), zinc oxide eugenol-based sealers (Tubliseal EWT, Pulp Canal Sealer EWT), calcium hydroxide-based sealer (Sealapex), and tricalcium phosphate based sealers (Sankin Apatite Root Canal Sealer I, II, III).

According to this study, the results were as follows:

The extracts of freshly mixed group showed higher toxicity than those of 24 h set group in MTT assay (p < 0.001).

CAPSEAL I and CAPSEAL II were less cytotoxic than AH 26, AH Plus, Tubliseal EWT, Pulp Canal Sealer EWT, Sealapex and SARCS II in freshly mixed group (p < 0.01).

AH 26 in freshly mixed group showed mutagenicity to TA98 and TA100 with and without S9 mix and AH Plus extracts also were mutagenic to TA100 with and without S9 mix.

Tubliseal EWT, Pulp Canal Sealer EWT and Sealapex in freshly mixed group were mutagenic to TA100 with S9 mix.

Among those of 24 h set groups, the extracts of SARCS II were mutagenic to TA98 with and without S9 mix and AH 26 showed mutagenic effects to TA98 with S9 mix.

No mutagenic effect of CAPSEAL I and CAPSEAL II was detected.

There is no statistically significant difference between CAPSEAL I and CAPSEAL II at MTT assay and Ames test in both freshly mixed group and 24 h set group.

Citations

Citations to this article as recorded by  
  • In vitrocytotoxicity of four calcium silicate-based endodontic cements on human monocytes, a colorimetric MTT assay
    Sedigheh Khedmat, Somayyeh Dehghan, Jamshid Hadjati, Farimah Masoumi, Mohammad Hossein Nekoofar, Paul Michael Howell Dummer
    Restorative Dentistry & Endodontics.2014; 39(3): 149.     CrossRef
  • A comparative evaluation of cytotoxicity of root canal sealers: anin vitrostudy
    Gautam Pyarelal Badole, Manjusha Madhukar Warhadpande, Ganesh Kothiramji Meshram, Rakesh Namdeoraoji Bahadure, Shubha Gopal Tawani, Gopal Tawani, Shital Gautam Badole
    Restorative Dentistry & Endodontics.2013; 38(4): 204.     CrossRef
  • Comparison of biocompatibility of four root perforation repair materials
    Min-Kyung Kang, In-Ho Bae, Jeong-Tae Koh, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh
    Journal of Korean Academy of Conservative Dentistry.2009; 34(3): 192.     CrossRef
  • 357 View
  • 1 Download
  • 3 Crossref
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