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The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells
Youngdan Jeong, Wonkyung Yang, Hyunjung Ko, Miri Kim
Restor Dent Endod 2014;39(3):187-194.   Published online June 19, 2014
DOI: https://doi.org/10.5395/rde.2014.39.3.187
AbstractAbstract PDFPubReaderePub
Objectives

The effects of bone morphogenetic protein-2 (BMP-2) and enamel matrix derivative (EMD) respectively with mineral trioxide aggregate (MTA) on hard tissue regeneration have been investigated in previous studies. This study aimed to compare the osteogenic effects of MTA/BMP-2 and MTA/EMD treatment in MC3T3-E1 cells.

Materials and Methods

MC3T3-E1 cells were treated with MTA (ProRoot, Dentsply), BMP-2 (R&D Systems), EMD (Emdogain, Straumann) separately and MTA/BMP-2 or MTA/EMD combination. Mineralization was evaluated by staining the calcium deposits with alkaline phosphatase (ALP, Sigma-Aldrich) and Alizarin red (Sigma-Aldrich). The effects on the osteoblast differentiation were evaluated by the expressions of osteogenic markers, including ALP, bone sialoprotein (BSP), osteocalcin (OCN), osteopontin (OPN) and osteonectin (OSN), as determined by reverse-transcription polymerase chain reaction analysis (RT-PCR, AccuPower PCR, Bioneer).

Results

Mineralization increased in the BMP-2 and MTA/BMP-2 groups and increased to a lesser extent in the MTA/EMD group but appeared to decrease in the MTA-only group based on Alizarin red staining. ALP expression largely decreased in the EMD and MTA/EMD groups based on ALP staining. In the MTA/BMP-2 group, mRNA expression of OPN on day 3 and BSP and OCN on day 7 significantly increased. In the MTA/EMD group, OSN and OCN gene expression significantly increased on day 7, whereas ALP expression decreased on days 3 and 7 (p < 0.05).

Conclusions

These results suggest the MTA/BMP-2 combination promoted more rapid differentiation in MC3T3-E1 cells than did MTA/EMD during the early mineralization period.

Citations

Citations to this article as recorded by  
  • Elucidating epigenetic mechanisms governing odontogenic differentiation in dental pulp stem cells: an in-depth exploration
    Lei Huang, Xuan Chen, Xiaoxia Yang, Yinchun Zhang, Yiyun Liang, Xiaoling Qiu
    Frontiers in Cell and Developmental Biology.2024;[Epub]     CrossRef
  • Evaluation of the genotoxicity, cytotoxicity, and bioactivity of calcium silicate-based cements
    Merve Esen, Yeliz Guven, Mehmet Fatih Seyhan, Handan Ersev, Elif Bahar Tuna-Ince
    BMC Oral Health.2024;[Epub]     CrossRef
  • GelMA‐based hydrogel biomaterial scaffold: A versatile platform for regenerative endodontics
    Lei Huang, Xuan Chen, XiaoXia Yang, Yinchun Zhang, Xiaoling Qiu
    Journal of Biomedical Materials Research Part B: Applied Biomaterials.2024;[Epub]     CrossRef
  • Experimental Validation of Antiobesogenic and Osteoprotective Efficacy of Ginsenoside CK via Targeting Lipid and Atherosclerosis Pathways
    Md. Niaj Morshed, Reshmi Akter, Imran Mahmud, Ah-Yeong Gwon, Jin Woo Jeang, Yeong-Geun Lee, Dae Won Park, Deok Chun Yang, Yeon Ju Kim, Se-Chan Kang
    Life.2024; 15(1): 41.     CrossRef
  • Anti-osteoporosis effects of triterpenoids from the fruit of sea buckthorn (Hippophae rhamnoides) through the promotion of osteoblast differentiation in mesenchymal stem cells, C3H10T1/2
    Da Eun Lee, Kun Hee Park, Joo-Hyun Hong, Seon Hee Kim, Ki-Moon Park, Ki Hyun Kim
    Archives of Pharmacal Research.2023; 46(9-10): 771.     CrossRef
  • In Silico and In Vitro Evaluation of Antiobesogenic and Osteoprotective Effect of Pomegranate Juice Fermented by Tannin Acyl Hydrolase and Lactobacillus vespulae DCY75 via the Wnt/β-Catenin Pathway
    Reshmi Akter, Vinothini Boopathi, Muhammad Awais, Juha Park, Byoung Man Kong, Se-Woung Oh, Ji-Hyung Oh, Jong Chan Ahn, Deok Chun Yang
    ACS Food Science & Technology.2023; 3(11): 1975.     CrossRef
  • Early induction of Hes1 by bone morphogenetic protein 9 plays a regulatory role in osteoblastic differentiation of a mesenchymal stem cell line
    Chang‐Hwan Seong, Norika Chiba, Mardiyantoro Fredy, Joji Kusuyama, Kiyohide Ishihata, Toshiro Kibe, Muhammad Subhan Amir, Ryohei Tada, Tomokazu Ohnishi, Norifumi Nakamura, Tetsuya Matsuguchi
    Journal of Cellular Biochemistry.2023; 124(9): 1366.     CrossRef
  • Effects of Fucoidan Powder Combined with Mineral Trioxide Aggregate as a Direct Pulp-Capping Material
    Mijoo Kim, Marc Hayashi, Bo Yu, Thomas K. Lee, Reuben H. Kim, Deuk-Won Jo
    Polymers.2022; 14(12): 2315.     CrossRef
  • Nerve growth factor promotes osteogenic differentiation of MC3T3-E1 cells via BMP-2/Smads pathway
    Xuming Yang, Donggang Mou, Qunying Yu, Jimei Zhang, Ying Xiong, Zhimin Zhang, Shan Xing
    Annals of Anatomy - Anatomischer Anzeiger.2022; 239: 151819.     CrossRef
  • Anti-Osteoporosis Effects of the Fruit of Sea Buckthorn (Hippophae rhamnoides) through Promotion of Osteogenic Differentiation in Ovariectomized Mice
    Kun Hee Park, Joo-Hyun Hong, Seon-Hee Kim, Jin-Chul Kim, Ki Hyun Kim, Ki-Moon Park
    Nutrients.2022; 14(17): 3604.     CrossRef
  • Oroactive dental biomaterials and their use in endodontic therapy
    Ebrahim Patel, Priyamvada Pradeep, Pradeep Kumar, Yahya E. Choonara, Viness Pillay
    Journal of Biomedical Materials Research Part B: Applied Biomaterials.2020; 108(1): 201.     CrossRef
  • BMP-2 and type I collagen preservation in human deciduous teeth after demineralization
    Nina Bono, Paolo Tarsini, Gabriele Candiani
    Journal of Applied Biomaterials & Functional Materials.2019;[Epub]     CrossRef
  • An assessment of the overexpression of BMP‐2 in transfected human osteoblast cells stimulated by mineral trioxide aggregate and Biodentine
    E. M. Rodrigues, A. L. Gomes‐Cornélio, A. Soares‐Costa, L. P. Salles, M. Velayutham, C. Rossa‐Junior, J. M. Guerreiro‐Tanomaru, M. Tanomaru‐Filho
    International Endodontic Journal.2017;[Epub]     CrossRef
  • Sandblasting and fibronectin-derived peptide immobilization on titanium surface increase adhesion and differentiation of osteoblast-like cells (MC3T3-E1)
    Samdharu Pramono, Kamolparn Pugdee, Jintamai Suwanprateep, Sittichai Koontongkaew
    Journal of Dental Sciences.2016; 11(4): 427.     CrossRef
  • Combined Effects of Growth Hormone and Mineral Trioxide Aggregate on Growth, Differentiation, and Angiogenesis in Human Dental Pulp Cells
    Hyung-Mun Yun, Seok-Woo Chang, Kyung-Ran Park, Lan Herr, Eun-Cheol Kim
    Journal of Endodontics.2016; 42(2): 269.     CrossRef
  • Combined effects of mineral trioxide aggregate and human placental extract on rat pulp tissue and growth, differentiation and angiogenesis in human dental pulp cells
    Seok-Woo Chang, Ji-Youn Kim, Mi-Joo Kim, Ga-Hyun Kim, Jin-Kyu Yi, Deok-Won Lee, Kee-Yeon Kum, Eun-Cheol Kim
    Acta Odontologica Scandinavica.2016; 74(4): 298.     CrossRef
  • Mineral trioxide aggregate induces osteoblastogenesis via Atf6
    Toyonobu Maeda, Atsuko Suzuki, Satoshi Yuzawa, Yuh Baba, Yuichi Kimura, Yasumasa Kato
    Bone Reports.2015; 2: 36.     CrossRef
  • Locally controlled delivery of TNFα antibody from a novel glucose-sensitive scaffold enhances alveolar bone healing in diabetic conditions
    Qi Wang, Hao Li, Yu Xiao, Shuan Li, Bo Li, Xiaowen Zhao, Lin Ye, Bin Guo, Xinmin Chen, Yi Ding, Chongyun Bao
    Journal of Controlled Release.2015; 206: 232.     CrossRef
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Enamel matrix derivative for replanted teeth in animal models: a systematic review and meta-analysis
Sahng G. Kim, Steven I. Ryu
Restor Dent Endod 2013;38(4):194-203.   Published online November 12, 2013
DOI: https://doi.org/10.5395/rde.2013.38.4.194
AbstractAbstract PDFPubReaderePub
Objectives

To investigate the effect of enamel matrix derivative (EMD) on periodontal healing of replanted teeth in animal models.

Materials and Methods

The authors searched MEDLINE, PubMed, EMBASE, Cochrane Library, Web of Knowledge and Scopus for articles published up to Oct 2012. Animal studies in which EMD was applied in transplanted or replanted teeth with adequate controls and histological data were considered. Normal periodontal healing or root resorption determined by histology after EMD was applied in replanted teeth with adequate controls was used as outcome measures. The following search strategy was used: ('Emdogain' OR 'enamel matrix proteins' OR 'enamel matrix derivative') AND ('avulsion' OR 'transplantion' OR 'autotransplantation' OR 'replantation').

Results

Six animal studies were included in the final review. There was great heterogeneity in study design among included studies. Two studies with similar study designs were identified and analyzed by a meta-analysis. The pooled estimates showed a significantly higher normal healing and surface resorption and significantly less inflammatory and replacement resorption in EMD-treated groups compared with non-EMD-treated groups.

Conclusions

With the limitations of this systematic review, the use of EMD led to greater normal periodontal healing and surface root resorption and less inflammatory and replacement root resorption in the presence of periodontal ligaments. However, no definite conclusion could be drawn with regard to the effect of EMD on periodontal healing and root resorption when no periodontal ligaments exist.

Citations

Citations to this article as recorded by  
  • Intentional Replantation of Failed Root Canal Treated Tooth
    Pritesh Kisanlal Agrawal, Narayan G. Jibhkate, Saurabh A. Redij
    Journal of Interdisciplinary Dentistry.2024; 14(2): 128.     CrossRef
  • Enamel matrix derivative in the treatment of tooth replantation: from a biological basis to clinical application
    Yao Lin, Liangping Chen, Yuling Xu, Mingwei Xu, Qinghua Liu, Junbing He
    Annals of Medicine.2024;[Epub]     CrossRef
  • Fibrillin protein, a candidate for creating a suitable scaffold in PDL regeneration while avoiding ankylosis
    Kyoko Oka
    genesis.2022;[Epub]     CrossRef
  • Can delayed grafting of frozen teeth achieve periodontal ligament healing?
    Yue Chen, Liang Chen, Min Zhou, Shouyin Yi, Juan Ran, Yuansi Long, Jing Luo, Kun Tian
    Medical Hypotheses.2022; 167: 110945.     CrossRef
  • Permanent tooth avulsion in children and adults: Therapeutic options for longer survival
    Simona Stojanović, Miloš Tijanić, Kristina Burić, Nina Burić, Milan Spasić, Kosta Todorović, Branislava Stojković, Marija Jovanović, Milica Petrović, Dušan Mitić
    Acta stomatologica Naissi.2021; 37(83): 2213.     CrossRef
  • Evidence mapping and quality assessment of systematic reviews in dental traumatology
    Nitesh Tewari, Vijay Prakash Mathur, Amandeep Kaur, Divesh Sardana, Morankar Rahul, Rigzen Tamchos, Priyanshi Ritwik, Shubhi Goel, Julie Schiavo
    Dental Traumatology.2021; 37(1): 17.     CrossRef
  • Application of Enamel Matrix Derivative (Emdogain) in Endodontic Therapy: A Comprehensive Literature Review
    Howard H. Wang, Nima D. Sarmast, Elham Shadmehr, Nikola Angelov, Shahrokh Shabahang, Mahmoud Torabinejad
    Journal of Endodontics.2018; 44(7): 1066.     CrossRef
  • Periodontal wound healing following reciprocal autologous root transplantation in class III furcation defects
    Naoshi Takeuchi, Yoshinori Shirakata, Yukiya Shinohara, Kotaro Sena, Kazuyuki Noguchi
    Journal of Periodontal & Implant Science.2017; 47(6): 352.     CrossRef
  • Effects of Fibrillin Application on Periodontal Ligament Regeneration in Mouse Model of Tooth Replantation
    Shougo Tamura, Kyoko Oka, Satoshi Itaya, Michiko Kira-Tatsuoka, Masako Toda, Arisa Higa, Masao Ozaki
    Journal of Hard Tissue Biology.2016; 25(3): 295.     CrossRef
  • Autotransplantation: a viable treatment option for adolescent patients with significantly compromised teeth
    D Ong, Y Itskovich, G Dance
    Australian Dental Journal.2016; 61(4): 396.     CrossRef
  • Influence of enamel matrix derivative on healing of root surfaces after bonding treatment and intentional replantation of vertically fractured roots
    Tsutomu Sugaya, Mahito Tomita, Youji Motoki, Hirofumi Miyaji, Masamitsu Kawamami
    Dental Traumatology.2016; 32(5): 397.     CrossRef
  • The effect of cathepsin K inhibitor on osteoclastic activity compared to alendronate and enamel matrix protein
    Wonkyung Yang, Hyunjung Ko, Heesun Kim, Miri Kim
    Dental Traumatology.2015; 31(3): 202.     CrossRef
  • The effects of bone morphogenetic protein-2 and enamel matrix derivative on the bioactivity of mineral trioxide aggregate in MC3T3-E1cells
    Youngdan Jeong, Wonkyung Yang, Hyunjung Ko, Miri Kim
    Restorative Dentistry & Endodontics.2014; 39(3): 187.     CrossRef
  • What is the Best Root Surface Treatment for Avulsed Teeth?
    Elif B Tuna , Duygu Yaman , Seiko Yamamato
    The Open Dentistry Journal.2014; 8(1): 175.     CrossRef
  • 374 View
  • 2 Download
  • 14 Crossref
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