Restorative Dentistry & Endodontics

Research Article

Evaluation of penetration depth of 2% chlorhexidine digluconate into root dentinal tubules using confocal laser scanning microscope: Sekar Vadhana, Jothi Latha, Natanasabapathy Velmurugan; Restor Dent Endod 2015;40(2):149-154. Published online March 4, 2015; DOI: https://doi.org/10.5395/rde.2015.40.2.149

Objectives

This study evaluated the penetration depth of 2% chlorhexidine digluconate (CHX) into root dentinal tubules and the influence of passive ultrasonic irrigation (PUI) using a confocal laser scanning microscope (CLSM).

Materials and Methods

Twenty freshly extracted anterior teeth were decoronated and instrumented using Mtwo rotary files up to size 40, 4% taper. The samples were randomly divided into two groups (n = 10), that is, conventional syringe irrigation (CSI) and PUI. CHX was mixed with Rhodamine B dye and was used as the final irrigant. The teeth were sectioned at coronal, middle and apical levels and viewed under CLSM to record the penetration depth of CHX. The data were statistically analyzed using Kruskal-Wallis and Mann-Whitney U tests.

Results

The mean penetration depths of 2% CHX in coronal, middle and apical thirds were 138 µm, 80 µm and 44 µm in CSI group, respectively, whereas the mean penetration depths were 209 µm, 138 µm and 72 µm respectively in PUI group. Statistically significant difference was present between CSI group and PUI group at all three levels (p < 0.01 for coronal third and p < 0.001 for middle and apical thirds). On intragroup analysis, both groups showed statistically significant difference among three levels (p < 0.001).

Conclusions

Penetration depth of 2% CHX into root dentinal tubules is deeper in coronal third when compared to middle and apical third. PUI aided in deeper penetration of 2% CHX into dentinal tubules when compared to conventional syringe irrigation at all three levels.

Citations

Citations to this article as recorded by

The ability of different diffusing enhancers to deliver chlorhexidine into dentinal tubules: An in vitro evaluation
Yi Luo, Mengting Duan, Runze Liu, Pei Liu, Wei Fan, Bing Fan
Journal of Dental Sciences.2024; 19(4): 2226. CrossRef
The effect of 2% chlorhexidine iontophoresis on dentin sealing ability of etch-and-rinse adhesive: An in vitro study
Kanittha Kijsamanmith, Panisara Srisatayasatien, Nichapa Thanindratarn, Chanisa Vichainarong, Jirapat Panyasukum
Journal of Dental Sciences.2024; 19(2): 846. CrossRef
Influence of different presentation forms of chlorhexidine on contaminated root canals during agitation
Ana B. S. Lopes, Augusto R. Lima, Juliana D. Bronzato, Daniel R. Herrera, Priscila A. Francisco, Maria C. C. Carvalho, Gabriel Abuna, Mario Sinhoreti, Brenda P. F. A. Gomes
Australian Endodontic Journal.2024; 50(3): 513. CrossRef
Evaluation of the transdentinal capability of the intrinsic antibacterial cetylpyridinium chloride/cholesterol sterosomes in vitro and in vivo
Xiaojun Yang, Chaoning Zhan, Tianjiao Cheng, Minchun Huang, Weiwen Ge, Yiqing Zhang, Ting Chen, Yanli Lu, Zhong‐Kai Cui, Jin Hou
International Endodontic Journal.2023; 56(2): 245. CrossRef
Dentinal tubule penetration of sodium hypochlorite in root canals with and without mechanical preparation and different irrigant activation methods
Renata Aqel de Oliveira, Theodoro Weissheimer, Gabriel Barcelos Só, Ricardo Abreu da Rosa, Matheus Albino Souza, Rodrigo Gonçalves Ribeiro, Marcus Vinicius Reis Só
Restorative Dentistry & Endodontics.2023;[Epub] CrossRef
Development of Lipidic Nanoplatform for Intra-Oral Delivery of Chlorhexidine: Characterization, Biocompatibility, and Assessment of Depth of Penetration in Extracted Human Teeth
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Nanomaterials.2022; 12(19): 3372. CrossRef
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Odontology.2021; 109(3): 687. CrossRef
The influence of irrigant activation, concentration and contact time on sodium hypochlorite penetration into root dentine: an ex vivo experiment
S. S. Virdee, D. J. J. Farnell, M. A. Silva, J. Camilleri, P. R. Cooper, P. L. Tomson
International Endodontic Journal.2020; 53(7): 986. CrossRef
Efficacy of different irrigation methods on dentinal tubule penetration of Chlorhexidine, QMix and Irritrol: A confocal laser scanning microscopy study
Meltem Küçük, Fatma Kermeoğlu
Australian Endodontic Journal.2019; 45(2): 202. CrossRef
Sodium hypochlorite penetration into dentinal tubules after manual dynamic agitation and ultrasonic activation: a histochemical evaluation
Luigi Generali, Erica Campolongo, Ugo Consolo, Carlo Bertoldi, Luciano Giardino, Francesco Cavani
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Push-out bond strength and dentinal tubule penetration of different root canal sealers used with coated core materials
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Original Article

Comparison of bond strength of a fiber post cemented with various resin cements: Hyun-A Lee, Young-Gon Cho; J Korean Acad Conserv Dent 2008;33(6):499-506. Published online November 30, 2008; DOI: https://doi.org/10.5395/JKACD.2008.33.6.499

Abstract PDF PubReader ePub

The purpose of this study was to compare the push-out strength of a fiber post cemented with various resin cements. Newly extracted 36 human mandibular premolars which had single root canal were selected and their crown portions were removed. The root canal was instrumented using PROTAPER™ system and obturated using continuous wave technique. In each root, a 9-mm deep post space was prepared. #2 translucent fiber post (DT Light post, Bisco Inc., Schaumburg, IL, U.S.A.) was cemented using injection technique with Uni-dose needle tip (Bisco) and six different resin cements. The tested resin cements were Duo-Link (Bisco Inc., Schaumburg, IL, U.S.A.), Variolink II (Ivoclar-Vivadent AG, Schann, Liechtenstein), Panavia F (Kuraray Medical Inc., Okayama, Japan), Multilink Automix (Ivoclar-Vivadent AG, Schann, Liechtenstein), RelyX Unicem (3M ESPE Dental Products, St. Paul, MN, U.S.A.), and Maxcem (Kerr Co., CA, U.S.A.). After storage in distilled water for 24 hours, each root was transversally sectioned into approximately 1-mm thick sections. This procedure resulted in 6 serial sections per root. Push-out test was performed using a universal testing machine (EZ Test, Shimadzu Co.) with a crosshead speed of 1 mm/min. The data were analyzed with one-way ANOVA and Tukey HSD (p=0.05).

The push-out strength of the groups which cemented fiber post with Panavia F and Multilink Automix were lower than those of the other groups. But, there were no statistically significant difference among groups at a probability level of 0.05.

Citations

Citations to this article as recorded by

Effect of surface treatments of fiber posts on bond strength to composite resin cores
Hye-Jo Keum, Hyun-Mi Yoo
Journal of Korean Academy of Conservative Dentistry.2010; 35(3): 173. CrossRef
Comparison of push-out bond strength of post according to cement application methods
Seo-Ryeong Kim, Jiwan Yum, Jeong-Kil Park, Bock Hur, Hyeon-Cheol Kim
Journal of Korean Academy of Conservative Dentistry.2010; 35(6): 479. CrossRef
Effect of dentin surface wetness on tensile bond strength of self adhesive resin cements
Sung-Young Yoon, Se-Hee Park, Jin-Woo Kim, Kyung-Mo Cho
Journal of Korean Academy of Conservative Dentistry.2009; 34(2): 113. CrossRef

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