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  • Original Article Effect of wetness on the enamel bonding
    Keun-Ho Ko, Young-Gon Cho, Cheul-Hee Jin, Sang-Hoon Yoo, Jong-Uk Kim, Byung-Cheul Park, Young-Jae Ki, Hee-Young Choi, Jong-Jin Kim
    2004;29(3):-211.
    DOI: https://doi.org/10.5395/JKACD.2004.29.3.205
    Published online: May 31, 2004

    Department of Conservative Dentistry, College of Dentistry, Chosun University, Korea.

    Corresponding author: Young-Gon Cho. Department of Conservative Dentistry, College of Dentistry, Chosun University, 421 Susuk-dong, Dong-gu, Gwangju, 501-825, Korea. Tel: 82-62-220-3840, Fax: 82-62-232-9064, ygcho@mail.chosun.ac.kr

    Copyright © 2004 Korean Academy of Conservative Dentistry

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    • This study evaluated the microleakage and interfacial gap between enamel and composite resin under the dry and wet condition of the enamel surface. V shaped class 5 cavities were prepared on the occlusal portion of extracted human molars. Samples were divided into three groups: D group (air dry for 10-15 s), BD group (blot dry with moist cotton pellet), and DR group (air dry for 10-15 s and rewet with Aqua-Prep F for 20 s).
      Cavities were filled using Aelitefil composite resin after applied One-Step. Microleakage was tested by 2% methylene blue dye solution and the data were statistically analysed by Kruskal-Wallis test and Mann-Whitney test. Also Enamel-resin interface was observed under SEM. Group BD showed statistically lower microleakage than group D (p < 0.05), but there was no statistically significant difference between group BD and DR (p > 0.05). At the enamel-resin interface, group D showed the gap of 2 µm thickness, but group BD and DR showed close adaptation.
      In conclusion, the use of blot dry and rewetting agent (Aqua-Prep F) resulted in decreased microleakage and improved adhesion between enamel and resin when using One-Step.
    Figure 1
    Number of microleakage scores of each group
    jkacd-29-205-g001.jpg
    Figure 2
    D (dry) group showed the gap (G) of 2 µm thickness at the enamel (E)-resin (R) interface (SEM ×5,000)
    jkacd-29-205-g002.jpg
    Figure 3
    BD (blot dry) group showed close adaptaition (CA) at the enamel (E)-resin (R) interface (SEM ×5,000)
    jkacd-29-205-g003.jpg
    Figure 4
    DR (dry/rewet with Aqua-Prep F) group showed close adaptation (CA) at the enamel (E)-resin (R) interface (SEM ×5,000)
    jkacd-29-205-g004.jpg
    Table 1
    Materials used in this study and their chemical composition

    BPDM, biphenyl dimethacrylate; Bis-GMA, bisphenol glycidyl methacrylate;

    HEMA, 2-hydroxyethyl methacrylate; TEGDMA, triethylenglycol dimethacrylate

    jkacd-29-205-i001.jpg
    Table 2
    Group classification according to the surface condition of cavity
    jkacd-29-205-i002.jpg
    Table 3
    Distribution of microleakage scores and means at enamel margins
    jkacd-29-205-i003.jpg
    Table 4
    Statistical analysis to enamel microleakage among groups by Kruskal-Wallis test and Mann-Whitney test

    *: significant differences (p < 0.05)

    jkacd-29-205-i004.jpg
    • 1. Amaral CM, Hara AT, Pimenta LAF, Rodrigues AL Jr. Microleakage of hydrophilic systems in class V composite restorations. Am J Dent. 2001;14(1):31-34.PubMed
    • 2. Gladys S, Van Meerbeek B, Lambrechets P, Vanherle G. Microleakage of adhesive restorative materials. Am J Dent. 2001;14(3):170-176.PubMed
    • 3. Fanchi M, Breschi L. Effects of acid-etching solutions on human enamel and dentin. Quintessence Int. 1995;26: 431-435.PubMed
    • 4. Kanca J. Resin bonding to wet substrate. I. Bonding to dentin. Quintessence Int. 1992;23(1):39-41.PubMed
    • 5. Moll K, Gartner T, Haller B. Effect of moist bonding on composite/enamel bond strength. Am J Dent. 2002;15(2):85-70.PubMed
    • 6. Perdigão J, Frankenberger R. Effect of solvent and rewetting time on dentin adhesion. Quintessence Int. 2001;32: 385-390.PubMed
    • 7. Swift EJ. Enamel and dentin bonding. J Esthet Dent. 2000;12(3):119-201.
    • 8. Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas M, Vijay P, Van Landuyt K, Lambrechts P, Vanherle G. Adhesion to enamel and dentin: Current status and future challenges. Oper Dent. 2003;28(3):215-235.PubMed
    • 9. Pashley DH, Ciucci B, Sano H, Horner JA. Permeability of dentin to adhesive agents. Quintessence Int. 1993;24: 618-631.PubMed
    • 10. Gwinnett AJ. Dentin bond strength after air drying and rewetting. Am J Dent. 1994;7(3):144-148.PubMed
    • 11. Tay FR, Gwinnett JA, Wei SHY. Micromorphological spectrum of acid conditioned dentin following the application of a water-based adhesive. Dent Mater. 1998;14: 329-338.PubMed
    • 12. Nakajima M, Kanemura N, Pereira PNR, Tagami J, Pashley DH. Comparative microtensile bond strength and SEM analysis of bonding to wet and dry dentin. Am J Dent. 2000;13(6):324-328.PubMed
    • 13. Gallo JR, Henderson M, Burgess JO. Shear bond strength to moist and dry dentin of four dentin bonding systems. Am J Dent. 2000;13(5):267-270.PubMed
    • 14. Pashley DH, Carvalho RM, Tay FR, Agee KA, Lee KW. Solvation of dried dentin matrix by water and other polar solvents. Am J Dent. 2002;15: 97-102.PubMed
    • 15. Braga RR, Cesar PF, Gonzaga CC. Tensile bond strength of filled and unfilled adhesives to dentin. Am J Dent. 2000;13(2):73-76.PubMed
    • 16. Gwinnett AJ. Moist vs. dry dentin: Its effect on shear bond strength. Am J Dent. 1992;5: 127-129.PubMed
    • 17. Kanca J 3rd. Effect of resin primer solvents and surface wetness on resin composite bond strength to dentin. Am J Dent. 1992;5: 213-215.PubMed
    • 18. Ritter AV, Heymann HO, Swift EJ, Perdigao J, Rosa BT. Effects of different re-wetting techniques on dentin shear bond strengths. J Esthet Dent. 2000;12(2):85-96.ArticlePubMed
    • 19. Perdigão J, Van Meerbeek B, Lopes MM, Ambrose WW. The effect of a re-wetting agent on dentin bonding. Dent Mater. 1999;15: 282-295.ArticlePubMed
    • 20. Pilo R, Cardash HS, Oz-Ari B, Ben-Amar A. Effect of preliminary treatment of the dentin surface on the shear bond strength of resin composite to dentin. Oper Dent. 2001;26: 569-575.PubMed
    • 21. Kanca J. Resin bonding to wet substrate. II. Bonding to enamel. Quintessence Int. 1992;23(9):625-627.PubMed
    • 22. Jain P, Stewart GP. Effect of dentin primer on shear bond strength of composite resin to moist and dry enamel. Oper Dent. 2000;25: 51-56.PubMed
    • 23. Gordan VV, Vargas MA, Denehy GE. Interfacial ultrastructure of the enamel/dentin bonding agents. Am J Dent. 1998;11: 13-16.PubMed
    • 24. Buonocore MG. Simple method of increasing the adhesion of acrylic filling materials to enamel surfaces. J Dent Res. 1955;28: 849-853.
    • 25. Abdalla AI, Garcia-Godoy F. Morphological characterization of single bottle adhesives and vital dentin interface. Am J Dent. 2002;15(1):31-34.PubMed
    • 26. Baratieri LN, Canabarro S, Lopes GC, Ritter AV. Effecct of resin viscosity and enamel beveling on the clinical performance of class V composite restorations: Three-year Results. Oper Dent. 2003;28(5):482-487.PubMed
    • 27. Barghi N, Knight GT, Berry TG. Comparing two methods of moisture control in bonding to enamel: A clinical study. Oper Dent. 1991;16: 130-135.PubMed
    • 28. Knight GT, Berry TG, Barghi N, Burns TR. Effects of two methods of moisture control on marginal microleakage between resin composite and etched enamel: a clinical study. Int J Prosthodont. 1993;6(5):475-479.PubMed
    • 29. Perdigão J, Swift EJ, Heymann HO, Malek MA. Effect of a re-wetting agent on the performance of acetone-based dentin adhesives. Am J Dent. 1998;11: 207-213.PubMed
    • 30. Tay FR, Gwinnett AJ, Wei SHY. Ultrastructure of the resin-dentin interface following reversible and irreversible rewetting. Am J Dent. 1997;10(2):77-82.PubMed
    • 31. Swift EJ. Resin desensitizers. J Esthet Dent. 1999;11(6):289-290.

    Tables & Figures

    Figure 1
    Number of microleakage scores of each group
    jkacd-29-205-g001.jpg
    Figure 2
    D (dry) group showed the gap (G) of 2 µm thickness at the enamel (E)-resin (R) interface (SEM ×5,000)
    jkacd-29-205-g002.jpg
    Figure 3
    BD (blot dry) group showed close adaptaition (CA) at the enamel (E)-resin (R) interface (SEM ×5,000)
    jkacd-29-205-g003.jpg
    Figure 4
    DR (dry/rewet with Aqua-Prep F) group showed close adaptation (CA) at the enamel (E)-resin (R) interface (SEM ×5,000)
    jkacd-29-205-g004.jpg
    Table 1
    Materials used in this study and their chemical composition

    BPDM, biphenyl dimethacrylate; Bis-GMA, bisphenol glycidyl methacrylate;

    HEMA, 2-hydroxyethyl methacrylate; TEGDMA, triethylenglycol dimethacrylate

    jkacd-29-205-i001.jpg
    Table 2
    Group classification according to the surface condition of cavity
    jkacd-29-205-i002.jpg
    Table 3
    Distribution of microleakage scores and means at enamel margins
    jkacd-29-205-i003.jpg
    Table 4
    Statistical analysis to enamel microleakage among groups by Kruskal-Wallis test and Mann-Whitney test

    *: significant differences (p < 0.05)

    jkacd-29-205-i004.jpg

    REFERENCES

    • 1. Amaral CM, Hara AT, Pimenta LAF, Rodrigues AL Jr. Microleakage of hydrophilic systems in class V composite restorations. Am J Dent. 2001;14(1):31-34.PubMed
    • 2. Gladys S, Van Meerbeek B, Lambrechets P, Vanherle G. Microleakage of adhesive restorative materials. Am J Dent. 2001;14(3):170-176.PubMed
    • 3. Fanchi M, Breschi L. Effects of acid-etching solutions on human enamel and dentin. Quintessence Int. 1995;26: 431-435.PubMed
    • 4. Kanca J. Resin bonding to wet substrate. I. Bonding to dentin. Quintessence Int. 1992;23(1):39-41.PubMed
    • 5. Moll K, Gartner T, Haller B. Effect of moist bonding on composite/enamel bond strength. Am J Dent. 2002;15(2):85-70.PubMed
    • 6. Perdigão J, Frankenberger R. Effect of solvent and rewetting time on dentin adhesion. Quintessence Int. 2001;32: 385-390.PubMed
    • 7. Swift EJ. Enamel and dentin bonding. J Esthet Dent. 2000;12(3):119-201.
    • 8. Van Meerbeek B, De Munck J, Yoshida Y, Inoue S, Vargas M, Vijay P, Van Landuyt K, Lambrechts P, Vanherle G. Adhesion to enamel and dentin: Current status and future challenges. Oper Dent. 2003;28(3):215-235.PubMed
    • 9. Pashley DH, Ciucci B, Sano H, Horner JA. Permeability of dentin to adhesive agents. Quintessence Int. 1993;24: 618-631.PubMed
    • 10. Gwinnett AJ. Dentin bond strength after air drying and rewetting. Am J Dent. 1994;7(3):144-148.PubMed
    • 11. Tay FR, Gwinnett JA, Wei SHY. Micromorphological spectrum of acid conditioned dentin following the application of a water-based adhesive. Dent Mater. 1998;14: 329-338.PubMed
    • 12. Nakajima M, Kanemura N, Pereira PNR, Tagami J, Pashley DH. Comparative microtensile bond strength and SEM analysis of bonding to wet and dry dentin. Am J Dent. 2000;13(6):324-328.PubMed
    • 13. Gallo JR, Henderson M, Burgess JO. Shear bond strength to moist and dry dentin of four dentin bonding systems. Am J Dent. 2000;13(5):267-270.PubMed
    • 14. Pashley DH, Carvalho RM, Tay FR, Agee KA, Lee KW. Solvation of dried dentin matrix by water and other polar solvents. Am J Dent. 2002;15: 97-102.PubMed
    • 15. Braga RR, Cesar PF, Gonzaga CC. Tensile bond strength of filled and unfilled adhesives to dentin. Am J Dent. 2000;13(2):73-76.PubMed
    • 16. Gwinnett AJ. Moist vs. dry dentin: Its effect on shear bond strength. Am J Dent. 1992;5: 127-129.PubMed
    • 17. Kanca J 3rd. Effect of resin primer solvents and surface wetness on resin composite bond strength to dentin. Am J Dent. 1992;5: 213-215.PubMed
    • 18. Ritter AV, Heymann HO, Swift EJ, Perdigao J, Rosa BT. Effects of different re-wetting techniques on dentin shear bond strengths. J Esthet Dent. 2000;12(2):85-96.ArticlePubMed
    • 19. Perdigão J, Van Meerbeek B, Lopes MM, Ambrose WW. The effect of a re-wetting agent on dentin bonding. Dent Mater. 1999;15: 282-295.ArticlePubMed
    • 20. Pilo R, Cardash HS, Oz-Ari B, Ben-Amar A. Effect of preliminary treatment of the dentin surface on the shear bond strength of resin composite to dentin. Oper Dent. 2001;26: 569-575.PubMed
    • 21. Kanca J. Resin bonding to wet substrate. II. Bonding to enamel. Quintessence Int. 1992;23(9):625-627.PubMed
    • 22. Jain P, Stewart GP. Effect of dentin primer on shear bond strength of composite resin to moist and dry enamel. Oper Dent. 2000;25: 51-56.PubMed
    • 23. Gordan VV, Vargas MA, Denehy GE. Interfacial ultrastructure of the enamel/dentin bonding agents. Am J Dent. 1998;11: 13-16.PubMed
    • 24. Buonocore MG. Simple method of increasing the adhesion of acrylic filling materials to enamel surfaces. J Dent Res. 1955;28: 849-853.
    • 25. Abdalla AI, Garcia-Godoy F. Morphological characterization of single bottle adhesives and vital dentin interface. Am J Dent. 2002;15(1):31-34.PubMed
    • 26. Baratieri LN, Canabarro S, Lopes GC, Ritter AV. Effecct of resin viscosity and enamel beveling on the clinical performance of class V composite restorations: Three-year Results. Oper Dent. 2003;28(5):482-487.PubMed
    • 27. Barghi N, Knight GT, Berry TG. Comparing two methods of moisture control in bonding to enamel: A clinical study. Oper Dent. 1991;16: 130-135.PubMed
    • 28. Knight GT, Berry TG, Barghi N, Burns TR. Effects of two methods of moisture control on marginal microleakage between resin composite and etched enamel: a clinical study. Int J Prosthodont. 1993;6(5):475-479.PubMed
    • 29. Perdigão J, Swift EJ, Heymann HO, Malek MA. Effect of a re-wetting agent on the performance of acetone-based dentin adhesives. Am J Dent. 1998;11: 207-213.PubMed
    • 30. Tay FR, Gwinnett AJ, Wei SHY. Ultrastructure of the resin-dentin interface following reversible and irreversible rewetting. Am J Dent. 1997;10(2):77-82.PubMed
    • 31. Swift EJ. Resin desensitizers. J Esthet Dent. 1999;11(6):289-290.

    Citations

    Citations to this article as recorded by  
    • Effect of an intermediate bonding resin and flowable resin on the compatibility of two-step total etching adhesives with a self-curing composite resin
      Sook-Kyung Choi, Ji-Wan Yum, Hyeon-Cheol Kim, Bock Hur, Jeong-Kil Park
      Journal of Korean Academy of Conservative Dentistry.2009; 34(5): 397.     CrossRef
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      J Korean Acad Conserv Dent. 2004;29(3):205-211.   Published online May 31, 2004
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    Figure
    • 1
    Effect of wetness on the enamel bonding
    Image Image Image Image
    Figure 1 Number of microleakage scores of each group
    Figure 2 D (dry) group showed the gap (G) of 2 µm thickness at the enamel (E)-resin (R) interface (SEM ×5,000)
    Figure 3 BD (blot dry) group showed close adaptaition (CA) at the enamel (E)-resin (R) interface (SEM ×5,000)
    Figure 4 DR (dry/rewet with Aqua-Prep F) group showed close adaptation (CA) at the enamel (E)-resin (R) interface (SEM ×5,000)
    Effect of wetness on the enamel bonding

    Materials used in this study and their chemical composition

    BPDM, biphenyl dimethacrylate; Bis-GMA, bisphenol glycidyl methacrylate;

    HEMA, 2-hydroxyethyl methacrylate; TEGDMA, triethylenglycol dimethacrylate

    Group classification according to the surface condition of cavity

    Distribution of microleakage scores and means at enamel margins

    Statistical analysis to enamel microleakage among groups by Kruskal-Wallis test and Mann-Whitney test

    *: significant differences (p < 0.05)

    Table 1 Materials used in this study and their chemical composition

    BPDM, biphenyl dimethacrylate; Bis-GMA, bisphenol glycidyl methacrylate;

    HEMA, 2-hydroxyethyl methacrylate; TEGDMA, triethylenglycol dimethacrylate

    Table 2 Group classification according to the surface condition of cavity

    Table 3 Distribution of microleakage scores and means at enamel margins

    Table 4 Statistical analysis to enamel microleakage among groups by Kruskal-Wallis test and Mann-Whitney test

    *: significant differences (p < 0.05)


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