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Effect of combined application of premixed bioceramic paste and diode laser in vital pulp therapy: an immunohistochemical randomized controlled split-mouth in vivo animal experiment
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Mo’men A. Salama, Dalia M. Fayyad, Mohamed I. Rabie, Manar A. A. Selim, Mahmoud F. Ahmed
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J Korean Acad Conserv Dent ;Published online December 8, 2025
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DOI: https://doi.org/10.5395/rde.2026.51.e4
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 Abstract
 PubReader ePub
- Objectives
This study aimed to evaluate the effect of premixed bioceramic paste (Well-Root PT; Vericom) compared to mineral trioxide aggregate (MTA) on the expression of the mineralization-related marker dentin sialoprotein (DSP) in dental pulp following direct pulp capping, with or without prior diode laser application.
Methods
Direct pulp exposures were performed in the upper and lower incisors of eight dogs (n = 96 teeth). Cavities (Class V) were created and received pulp capping with either Well-Root PT (n = 32), MTA (n = 32), or no capping material (Teflon disc only) (n = 32), with or without the application of a diode laser. Immunohistochemical analysis of DSP expression was conducted and quantified as the mean area percentage using ImageJ software at 2 and 8 weeks posttreatment.
Results
Both the Well-Root PT and MTA groups showed significantly increased DSP expression compared to the control group at both 2 and 8 weeks (p < 0.05). No significant difference in the mean area percentage of DSP expression was found between the Well-Root PT and MTA groups. The diode laser application did not produce a significant effect on DSP expression. Within-group comparison revealed a significant increase in DSP expression between the 2- and 8-week follow-up periods (p < 0.05).
Conclusions
Well-Root PT demonstrated comparable efficacy to MTA in promoting DSP expression, supporting its use as an effective direct pulp capping material. Diode laser application prior to capping had no effect on DSP expression in this experimental model.
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KACD
