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Research Article
Effects of proanthocyanidin, a crosslinking agent, on physical and biological properties of collagen hydrogel scaffold
Yoorina Choi, Hee-Jin Kim, Kyung-San Min
Restor Dent Endod 2016;41(4):296-303.   Published online October 4, 2016
DOI: https://doi.org/10.5395/rde.2016.41.4.296
AbstractAbstract PDFPubReaderePub
Objectives

The purpose of the present study was to evaluate the effects of proanthocyanidin (PAC), a crosslinking agent, on the physical properties of a collagen hydrogel and the behavior of human periodontal ligament cells (hPDLCs) cultured in the scaffold.

Materials and Methods

Viability of hPDLCs treated with PAC was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The physical properties of PAC treated collagen hydrogel scaffold were evaluated by the measurement of setting time, surface roughness, and differential scanning calorimetry (DSC). The behavior of the hPDLCs in the collagen scaffold was evaluated by cell morphology observation and cell numbers counting.

Results

The setting time of the collagen scaffold was shortened in the presence of PAC (p < 0.05). The surface roughness of the PAC-treated collagen was higher compared to the untreated control group (p < 0.05). The thermogram of the crosslinked collagen exhibited a higher endothermic peak compared to the uncrosslinked one. Cells in the PAC-treated collagen were observed to attach in closer proximity to one another with more cytoplasmic extensions compared to cells in the untreated control group. The number of cells cultured in the PAC-treated collagen scaffolds was significantly increased compared to the untreated control (p < 0.05).

Conclusions

Our results showed that PAC enhanced the physical properties of the collagen scaffold. Furthermore, the proliferation of hPDLCs cultured in the collagen scaffold crosslinked with PAC was facilitated. Conclusively, the application of PAC to the collagen scaffold may be beneficial for engineering-based periodontal ligament regeneration in delayed replantation.

Citations

Citations to this article as recorded by  
  • Effect of collagen crosslinkers on sodium hypochlorite treated dentin bond strength: a systematic review and meta-analysis
    Weiqing Zhou, Shuting Feng, Xiaojun Chu, Shuaimei Xu, Xiongqun Zeng
    Frontiers in Bioengineering and Biotechnology.2025;[Epub]     CrossRef
  • A highly biocompatible CE-crosslinked collagen implant with exceptional anti-calcification and collagen regeneration capabilities for aging skin rejuvenation
    Qi Wang, Huiyu Yan, Linyan Yao, Wenhua Li, Jianxi Xiao
    Journal of Materials Chemistry B.2024; 12(18): 4467.     CrossRef
  • Dexamethasone release from hyaluronic acid microparticle and proanthocyanidin-gelatin hydrogel in sciatic tissue regeneration
    Kazem Javanmardi, Hamideh Shahbazi, Ava Soltani Hekmat, Mehdi Khanmohammadi, Arash Goodarzi
    Journal of Materials Science: Materials in Medicine.2024;[Epub]     CrossRef
  • New Materials Based on Collagen and Taxifolin Derivatives: Production and Properties
    Yu. V. Shatalin, M. I. Kobyakova, V. S. Shubina
    Биологические мембраны Журнал мембранной и клеточной биологии.2024; 41(1): 82.     CrossRef
  • Modulation of Adhesion and Migration of NIH/3T3 Cells in Collagen Materials by Taxifolin Derivatives
    Yu. V. Shatalin, M. I. Kobyakova, V. S. Shubina
    Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology.2023; 17(S1): S85.     CrossRef
  • Development and characterization of crosslinked k-carrageenan/sericin blend with covalent agents or thermal crosslink for indomethacin extended release
    Wedja Timóteo Vieira, Meuris Gurgel Carlos da Silva, Laura de Oliveira Nascimento, Melissa Gurgel Adeodato Vieira
    International Journal of Biological Macromolecules.2023; 246: 125558.     CrossRef
  • New Challenges and Prospective Applications of Three-Dimensional Bioactive Polymeric Hydrogels in Oral and Craniofacial Tissue Engineering: A Narrative Review
    Gamal Abdel Nasser Atia, Hany K. Shalaby, Naema Goda Ali, Shaimaa Mohammed Morsy, Mohamed Mohamady Ghobashy, Hager Abdel Nasser Attia, Paritosh Barai, Norhan Nady, Ahmad S. Kodous, Hasi Rani Barai
    Pharmaceuticals.2023; 16(5): 702.     CrossRef
  • Polyphenols: Bioavailability, Microbiome Interactions and Cellular Effects on Health in Humans and Animals
    Michael B. Scott, Amy K. Styring, James S. O. McCullagh
    Pathogens.2022; 11(7): 770.     CrossRef
  • Advances of Hydrogel Therapy in Periodontal Regeneration—A Materials Perspective Review
    Maoxue Li, Jiaxi Lv, Yi Yang, Guoping Cheng, Shujuan Guo, Chengcheng Liu, Yi Ding
    Gels.2022; 8(10): 624.     CrossRef
  • Collagen stabilization by natural cross-linkers: A qualitative and quantitative FTIR study on ultra-thin dentin collagen model
    Rong WANG, Tyler STANLEY, Xiaomei YAO, Hang LIU, Yong WANG
    Dental Materials Journal.2022; 41(3): 440.     CrossRef
  • Cross-Linking Agents for Electrospinning-Based Bone Tissue Engineering
    Dong-Jin Lim
    International Journal of Molecular Sciences.2022; 23(10): 5444.     CrossRef
  • Dense lamellar scaffold, biomimetically inspired, for reverse cardiac remodeling: Effect of proanthocyanidins and glutaraldehyde
    Thais Alves, Juliana Ferreira Souza, Venancio Alves Amaral, Alessandra Candida Rios, Tais Costa, Kessi Crescencio, Fernando Batain, Denise Grotto, Renata Lima, Lindemberg Silveira Filho, Jose Oliveira Junior, Patricia Severino, Norberto Aranha, Marco Chau
    Journal of Dispersion Science and Technology.2021; 42(2): 248.     CrossRef
  • The effect of the cross-linker ratio used in gellan gum biomaterial synthesis on biomineralization
    Serbülent TÜRK, Burak ÜNLÜ, Mahmut ÖZACAR
    Bulletin of Biotechnology.2021; 2(2): 27.     CrossRef
  • The recent advances in scaffolds for integrated periodontal regeneration
    Hyun Nyun Woo, Young Joon Cho, Solaiman Tarafder, Chang H. Lee
    Bioactive Materials.2021; 6(10): 3328.     CrossRef
  • Plant based cross-linkers for tissue engineering applications
    Abhishek Indurkar, Ashish Pandit, Ratnesh Jain, Prajakta Dandekar
    Journal of Biomaterials Applications.2021; 36(1): 76.     CrossRef
  • Plant-based biomaterials in tissue engineering
    Abhishek Indurkar, Ashish Pandit, Ratnesh Jain, Prajakta Dandekar
    Bioprinting.2021; 21: e00127.     CrossRef
  • Traditional Chinese Medicine and orthopedic biomaterials: Host of opportunities from herbal extracts
    Huijuan Tang, Andrell Hosein, Monica Mattioli-Belmonte
    Materials Science and Engineering: C.2021; 120: 111760.     CrossRef
  • Adsorption of Gold Ions onto Sericin and Alginate Particles Chemically Crosslinked by Proanthocyanidins: a Complete Fixed-Bed Column Study
    Nilza Tatiane das Graças Santos, Richard Landers, Meuris Gurgel Carlos da Silva, Melissa Gurgel Adeodato Vieira
    Industrial & Engineering Chemistry Research.2020; 59(1): 318.     CrossRef
  • Proanthocyanidin as a crosslinking agent for fibrin, collagen hydrogels and their composites with decellularized Wharton’s-jelly-extract for tissue engineering applications
    Elham Hasanzadeh, Narges Mahmoodi, Arefeh Basiri, Faezeh Esmaeili Ranjbar, Zahra Hassannejad, Somayeh Ebrahimi-Barough, Mahmoud Azami, Jafar Ai, Vafa Rahimi-Movaghar
    Journal of Bioactive and Compatible Polymers.2020; 35(6): 554.     CrossRef
  • Hydrogels for the Delivery of Plant-Derived (Poly)Phenols
    Nicola Micale, Andrea Citarella, Maria Sofia Molonia, Antonio Speciale, Francesco Cimino, Antonella Saija, Mariateresa Cristani
    Molecules.2020; 25(14): 3254.     CrossRef
  • Natural biopolymer‐based hydrogels for use in food and agriculture
    Miri Klein, Elena Poverenov
    Journal of the Science of Food and Agriculture.2020; 100(6): 2337.     CrossRef
  • Grape Seed-Inspired Smart Hydrogel Scaffolds for Melanoma Therapy and Wound Healing
    Hongshi Ma, Quan Zhou, Jiang Chang, Chengtie Wu
    ACS Nano.2019; 13(4): 4302.     CrossRef
  • Improvement of the Physical Properties of Guided Bone Regeneration Membrane from Porcine Pericardium by Polyphenols-Rich Pomace Extract
    Nazario Russo, Clara Cassinelli, Elisa Torre, Marco Morra, Giorgio Iviglia
    Materials.2019; 12(16): 2564.     CrossRef
  • Novel Biomedical Applications of Crosslinked Collagen
    Lisha Gu, Tiantian Shan, Yu-xuan Ma, Franklin R. Tay, Lina Niu
    Trends in Biotechnology.2019; 37(5): 464.     CrossRef
  • The prospects of collagen as a basis for curable and activated osteoplastic materials
    N. L. Fatkhudinova, A. V. Vasilyev, T. B. Bukharova, E. O. Osidak, N. V. Starikova, S. P. Domogatsky, D. V. Goldshtein, A. A. Kulakov
    Stomatologiya.2018; 97(6): 78.     CrossRef
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Basic Researchs
Biocompatibility of bioaggregate cement on human pulp and periodontal ligament (PDL) derived cells
Choo-Ryung Chung, Euiseong Kim, Su-Jung Shin
J Korean Acad Conserv Dent 2010;35(6):473-478.   Published online November 30, 2010
DOI: https://doi.org/10.5395/JKACD.2010.35.6.473
AbstractAbstract PDFPubReaderePub
Objectives

This study was performed to investigate the biocompatibility of newly introduced Bioaggregate on human pulp and PDL cells.

Materials and Methods

Cells were collected from human pulp and PDL tissue of extracted premolars. Cell culture plate was coated either with Bioaggregate or white MTA, then the same number of cells were poured to cell culture dishes. Cell attachment and growth was examined under a phase microscope after 1,3 and 7 days of seeding. Cell viability was measured and the data was analyzed using Student t-test and one way ANOVA.

Results

Both types of cells used in this study were well attached and grew healthy on Bioaggregate and MTA coated culture dishes. No cell inhibition zone was observed in Bioaggregate group. There was no statistical difference of viable cells between bioaggreagte and MTA groups.

Conclusions

Bioaggregate appeared to be biocompatible compared with white MTA on human pulp and PDL cells.

Citations

Citations to this article as recorded by  
  • Evaluation of bioactivity, biocompatibility, and antibacterial properties of tricalcium silicate bone cement modified with wollastonite/ fluorapatite glass and glass-ceramic
    H.K. Abd El-Hamid, A.M. Fayad, R.L. Elwan
    Ceramics International.2024; 50(14): 25322.     CrossRef
  • Influence of insulin on the healing of exposed dental pulp after pulp capping: An experimental study in a dog model
    Mokhtar A. Al‐Anesi, Ashraf M. Abu‐Seida, Salma H. El Ashry, Abeer H. Mahran, Ehab S. Abd‐Elhamid
    Special Care in Dentistry.2021; 41(1): 49.     CrossRef
  • ROOT END FILLING MATERIALS – A REVIEW
    Bynagari Chandra Shekar, Veerendra Uppin, Madhu Pujar
    GLOBAL JOURNAL FOR RESEARCH ANALYSIS.2021; : 5.     CrossRef
  • Effects of two fast-setting calcium-silicate cements on cell viability and angiogenic factor release in human pulp-derived cells
    Chooryung J. Chung, Euiseong Kim, Minju Song, Jeong-Won Park, Su-Jung Shin
    Odontology.2016; 104(2): 143.     CrossRef
  • Cytotoxicity and physical properties of tricalcium silicate-based endodontic materials
    Young-Eun Jang, Bin-Na Lee, Jeong-Tae Koh, Yeong-Joon Park, Nam-Eok Joo, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Yun-Chan Hwang
    Restorative Dentistry & Endodontics.2014; 39(2): 89.     CrossRef
  • Biocompatibility of root-end filling materials: recent update
    Payal Saxena, Saurabh Kumar Gupta, Vilas Newaskar
    Restorative Dentistry & Endodontics.2013; 38(3): 119.     CrossRef
  • 341 View
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  • 6 Crossref
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The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure
Jin-Ho Chung, Jin Kim, Seong-Ho Choi, Eui-Seong Kim, Jiyong Park, Seung-Jong Lee
J Korean Acad Conserv Dent 2010;35(4):285-294.   Published online July 31, 2010
DOI: https://doi.org/10.5395/JKACD.2010.35.4.285
AbstractAbstract PDFPubReaderePub

The purpose of this study was to evaluate the viability of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure by means of MTT assay, WST-1 assay. 12 teeth of Sprague-Dawley white female rats of 4 week-old were used for each group.

Both side of the first and second maxillary molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group 1 (Immediate extraction), group 2 (Slow freezing under pressure of 3 MPa), group 3 (Slow freezing under pressure of 2 MPa), group 4 (Slow freezing under no additional pressure), group 5 (Rapid freezing in liquid nitrogen under pressure of 2 MPa), group 6 (Rapid freezing in liquid nitrogen under no additional pressure), group 7 (low-temperature preservation at 0℃ under pressure of 2 MPa), group 8 (low-temperature preservation at 0℃ under no additional pressure), group 9 (low-temperature preservation at -5℃ under pressure of 90 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37℃ water bath, then MTT assay, WST-1 assay were processed. One way ANOVA and Tukey HSD method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization.

In both MTT and WST-1 assay, group 7 (0℃/2 MPa) showed higher viability of periodontal ligament cells than other group (2-6, 8) and this was statistically significant (p < 0.05), but showed lower viability than group 1, immediate extraction group (no statistical significance).

By the results of this study, low-temperature preservation at 0℃ under pressure of 2 MPa suggest the possibility for long term preservation of teeth.

Citations

Citations to this article as recorded by  
  • Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
    Sun Mi Jang, Sin-Yeon Cho, Eui-Seong Kim, Il-Young Jung, Seung Jong Lee
    Journal of Korean Dental Science.2016; 9(1): 1.     CrossRef
  • 294 View
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  • 1 Crossref
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Original Articles
The comparison of gene expression from human dental pulp cells and periodontal ligament cells
Hyoun So, Sang-Hyuk Park, Gi-Woon Choi
J Korean Acad Conserv Dent 2009;34(5):430-441.   Published online September 30, 2009
DOI: https://doi.org/10.5395/JKACD.2009.34.5.430
AbstractAbstract PDFPubReaderePub

The purpose of this study was to characterize functional distinction between human dental pulp cells(PC) and periodontal ligament cells(PDLC) using cDNA microarray assay and to confirm the results of the microarray assay using RT-PCR. 3 genes out of 51 genes which were found to be more expressed(>2 fold) in PC were selected, and 3 genes out of 19 genes which were found to be more expressed(>2 fold) in PDLC were selected for RT-PCR as well.

According to this study, the results were as follows:

1. From the microarray assay, 51 genes were more expressed (2 fold) from PC than PDLC.

2. RT-PCR confirmed that ITGA4 and TGF β2 were more expressed in PC than in PDLC.

3. From the microarray assay, 19 genes were more expressed (2 fold) from PDLC than PC.

4. RT-PCR confirmed that LUM, WISP1, and MMP1 were more expressed in PDLC than in PC.

From the present study, different expression of the genes between the PC and PDLC were characterized to show the genes which play an important role in dentinogenesis were more expressed from PC than PDLC, while the genes which were related with collagen synthesis were more expressed from PDLC than PC.

Citations

Citations to this article as recorded by  
  • Gene expression profiling in human dental pulp cells treated with mineral trioxide aggregate
    Yong-Beom Kim, Won-Jun Shon, WooCheol Lee, Kee-Yeon Kum, Seung-Ho Baek, Kwang-Shik Bae
    Journal of Korean Academy of Conservative Dentistry.2010; 35(3): 152.     CrossRef
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  • 1 Crossref
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THE EFFICACY OF PROGRAMMED CRYO-PRESERVATION UNDER PRESSURE IN RAT PERIODONTAL LIGAMENT CELLS
Young-Eun Lee, Eui-Seong Kim, Jin Kim, Seung-Hoon Han, Seung-Jong Lee
J Korean Acad Conserv Dent 2009;34(4):356-363.   Published online January 14, 2009
DOI: https://doi.org/10.5395/JKACD.2009.34.4.356
AbstractAbstract PDFPubReaderePub
Abstract

The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group.

Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at 4°C for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in 37°C water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization.

In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group.

By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth.

Citations

Citations to this article as recorded by  
  • Effects of Slow Programmable Cryopreservation on Preserving Viability of the Cultured Periodontal Ligament Cells from Human Impacted Third Molar
    Jin-Woo Kim, Tae-Yi Kim, Ye-mi Kim, Eun-Kyoung Pang, Sun-Jong Kim
    Journal of Korean Dental Science.2015; 8(2): 57.     CrossRef
  • The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure
    Jin-Ho Chung, Jin Kim, Seong-Ho Choi, Eui-Seong Kim, Jiyong Park, Seung-Jong Lee
    Journal of Korean Academy of Conservative Dentistry.2010; 35(4): 285.     CrossRef
  • Comparison of viability of oral epithelial cells stored by different freezing methods
    Do-Young Baek, Seung-Jong Lee, Han-Sung Jung, EuiSeong Kim
    Journal of Korean Academy of Conservative Dentistry.2009; 34(6): 491.     CrossRef
  • 290 View
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Evaluation of the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field
Hyun-Jung Ahn, Eui-Seong Kim, Jin Kim, Duck-Won Kim, Ki-Yeol Kim, Chan-Young Lee, Seung-Jong Lee
J Korean Acad Conserv Dent 2008;33(4):332-340.   Published online July 31, 2008
DOI: https://doi.org/10.5395/JKACD.2008.33.4.332
AbstractAbstract PDFPubReaderePub

The purpose of this study was to evaluate the viability of periodontal ligament cell in rat teeth using slow cryopreservation method with magnetic field through MTT assay and TUNEL test. For each group, 12 teeth of 4 weeks old white female Sprague-Dawley rat were used for MTT assay, and 6 teeth in TUNEL test. The Maxillary left and right, first and second molars were extracted as atraumatically as possible under tiletamine anesthesia. The experimental groups were group1 (immediately extraction), group 2 (cold preservation at 4℃ for 1 week), group 3 (rapid cryopreservation in liquid nitrogen), group 4 (slow cryopreservation with magnetic field of 1 G), and group 5 (slow cryopreservation). F medium was used as preservation medium and 10% DMSO as cryoprotectant. After preservation and thawing, the MTT assay and TUNEL test were processed. One way ANOVA and Scheffe method were performed at the 95% level of confidence. The value of optical density obtained after MTT analysis was divided by the value of eosin staining for tissue volume standardization. In both MTT assay and TUNEL test, it had showed no significant difference among group 3, 4, and 5. And group 3 had showed higher viability of periodontal ligament cell than group 2.

From this study, slow cryopreservation method with magnetic field can be used as one of cryopreservation methods.

Citations

Citations to this article as recorded by  
  • The evaluation of periodontal ligament cells of rat teeth after low-temperature preservation under high pressure
    Jin-Ho Chung, Jin Kim, Seong-Ho Choi, Eui-Seong Kim, Jiyong Park, Seung-Jong Lee
    Journal of Korean Academy of Conservative Dentistry.2010; 35(4): 285.     CrossRef
  • Comparison of viability of oral epithelial cells stored by different freezing methods
    Do-Young Baek, Seung-Jong Lee, Han-Sung Jung, EuiSeong Kim
    Journal of Korean Academy of Conservative Dentistry.2009; 34(6): 491.     CrossRef
  • The efficacy of programmed cryo-preservation under pressure in rat periodontal ligament cells
    Young-Eun Lee, Eui-Seong Kim, Jin Kim, Seung-Hoon Han, Seung-Jong Lee
    Journal of Korean Academy of Conservative Dentistry.2009; 34(4): 356.     CrossRef
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Evaluation of the radiopacity and cytotoxicity of resinous root canal sealers
Chang-Kyu Kim, Hyun-Wook Ryu, Hoon-Sang Chang, Byung-Do Lee, Kyung-San Min, Chan-Ui Hong
J Korean Acad Conserv Dent 2007;32(5):419-425.   Published online September 30, 2007
DOI: https://doi.org/10.5395/JKACD.2007.32.5.419
AbstractAbstract PDFPubReaderePub

The aim of this study was to evaluate the radiopacity and cytotoxicity of three resin-based (AH 26, EZ fill and AD Seal), a zinc oxide-eugenol-based (ZOB Seal), and a calcium hydroxide-based (Sealapex) root canal sealers. Specimens, 10 mm in diameter and 1 mm in thickness, were radiographed simultaneously with an aluminum step wedge using occlusal films, according to ISO 6876/2001 standards. Radiographs were digitized, and the radiopacity of sealers was compared to the different thicknesses of the aluminum step wedge, using the Scion image software. Using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, the cytotoxicity of each material was determined in immortalized human periodontal ligament (IPDL) cells.

The results demonstrated that EZ fill was the most radiopaque sealer, while Sealapex was the least radiopaque (p < 0.05). AH 26, AD Seal and ZOB Seal presented intermediate radiopacity values. All the materials evaluated, except for Sealapex, presented the minimum radiopacity required by ISO standards. The cell viabilities of resin-based root canal sealers were statistically higher than that of other type of root canal sealers through the all experimental time. Further, EZ fill showed statistically lower cell viability in 24 and 48 hours compared to AD Seal and in 72 hours compared to all other resin-based root canal sealers. However, there was no correlation between the radiopacity and cytotoxicity of three resin-based root canals sealers (p > 0.05).

These results indicate that resin-based root canal sealer is more biocompatible and has advantage in terms of radiopacity.

Citations

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  • A Comparative Evaluation of Two Commonly Used GP Solvents on Different Epoxy Resin-based Sealers: An In Vitro Study
    Sakshi Tyagi, Ekta Choudhary, Rajat Chauhan, Ashish Choudhary
    International Journal of Clinical Pediatric Dentistry.2020; 13(1): 35.     CrossRef
  • Evaluation of softening ability of Xylene & Endosolv-R on three different epoxy resin based sealers within 1 to 2 minutes - anin vitrostudy
    Pratima Ramakrishna Shenoi, Gautam Pyarelal Badole, Rajiv Tarachand Khode
    Restorative Dentistry & Endodontics.2014; 39(1): 17.     CrossRef
  • A comparative evaluation of cytotoxicity of root canal sealers: anin vitrostudy
    Gautam Pyarelal Badole, Manjusha Madhukar Warhadpande, Ganesh Kothiramji Meshram, Rakesh Namdeoraoji Bahadure, Shubha Gopal Tawani, Gopal Tawani, Shital Gautam Badole
    Restorative Dentistry & Endodontics.2013; 38(4): 204.     CrossRef
  • Evaluation of radiopacity and discriminability of various fiber reinforced composite posts
    Eun-Hye Lee, Hang-Moon Choi, Se-Hee Park, Jin-Woo Kim, Kyung-Mo Cho
    Journal of Korean Academy of Conservative Dentistry.2010; 35(3): 188.     CrossRef
  • A comparative study on radiopacity of root canal sealers
    Tae-Min Kim, Seo-Kyoung Kim, In-Nam Hwang, Yun-Chan Hwang, Byung-Cheol Kang, Suk-Ja Yoon, Jae-Seo Lee, Won-Mann Oh
    Journal of Korean Academy of Conservative Dentistry.2009; 34(1): 61.     CrossRef
  • A comparative study on radiopacity of canal filling and retrograde root-end filling materials
    Yong-Sang Kim, Seo-Kyong Kim, Yun-Chan Hwang, In-Nam Hwang, Won-Mann Oh
    Journal of Korean Academy of Conservative Dentistry.2008; 33(2): 107.     CrossRef
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Evaluation of periodontal ligament cell viability in rat teeth according to various extra-oral dry storage times using MTT assay
In-Soo Jeon, Eui-Seong Kim, Jin Kim, Seung-Jong Lee
J Korean Acad Conserv Dent 2006;31(5):398-408.   Published online September 30, 2006
DOI: https://doi.org/10.5395/JKACD.2006.31.5.398
AbstractAbstract PDFPubReaderePub

The purpose of this study was to verify the usefulness of MTT analysis as a tool of measurement of the periodontal ligament cell viability from the extracted rat molar.

A total of 80 Sprague-Dawley white female rat of 4 week-old with a body weight of 100 grams were used. The maxillary left and right, first and second molars were extracted under Ketamine anesthesia. Twenty-four teeth of each group (divided as five groups depending upon the time-lapse after extraction such as immediate, 10, 20, 40 and 60 minutes) were immersed in 200 µl of MTT solution (0.5 mg/ml) and processed for optical density measurements . Another 10 teeth of each group were treated as same as above and sectioned at 10 µm for microscopic examination.

All measurements values were divided by the value of hematoxylin-eosin staining which represented the volume of each corresponding samples. Immediate and 10 minute groups showed highest MTT values followed by 20, 40, and 60 minutes consecutively. Statistical significance (p < 0.05) existed between all groups except in immediate versus 10 minute groups and 40 versus 60 minutes. Histological findings also showed similar findings with MTT results in crystal shape and crystal numbers between the experimental groups.

These data indicate that in vivo MTT analysis may be of value for evaluation of the periodontal ligament cell viability without time- consuming cell culturing processes.

Citations

Citations to this article as recorded by  
  • Evaluation of the Viability of Rat Periodontal Ligament Cells after Storing at 0℃/2 MPa Condition up to One Week: In Vivo MTT Method
    Sun Mi Jang, Sin-Yeon Cho, Eui-Seong Kim, Il-Young Jung, Seung Jong Lee
    Journal of Korean Dental Science.2016; 9(1): 1.     CrossRef
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Evaluation of periodontal ligament cell viability in rat teeth after frozen preservation using in-vivo MTT assay
Jae-Wook Kim, Eui-Sung Kim, Jin Kim, Seung-Jong Lee
J Korean Acad Conserv Dent 2006;31(3):192-202.   Published online May 31, 2006
DOI: https://doi.org/10.5395/JKACD.2006.31.3.192
AbstractAbstract PDFPubReaderePub

The purpose of this study was to examine the viability of PDL cells in rat molars by using in vivo MTT assay, which was used to compare fast cryopreservation group by liquid nitrogen (-196℃) with 4℃ cold preservation group.

A total of 74 Sprague-Dawley white female rats of 4 week-old with a body weight of 100 grams were used. The maxillary left and right, first and second molars were extracted as atraumatically as possible under ketamine anesthesia.

Ten teeth of each group were divided as six experimental groups depending upon the preservation. Cryopreservation groups were Group 1 (5% DMSO 6% HES in F medium), Group 2 (10% DMSO in F medium), Group 3 (5% DMSO 6% HES in Viaspan®), Group 4 (10% DMSO in Viaspan®) which were cryopreserved for 1 week and cold preservation groups were Group 5 (F medium), Group 6 (Viaspan®) at 4℃ for 1 week. Immediate extraction group was used as a control. After preservation and thawing, the in vivo MTT assay was processed. Two way ANOVA and Duncan's Multiple Range Test was performed at the 95% level of confidence. Another 2 teeth of each group were treated as the same manner and frozen sections 10 µm thick for microscopic observation.

The value of optical density obtained after in vivo MTT analysis was divided by the value of eosin staining for tissue volume standardization. Group 1, 2 had significantly higher optical density than Group 3 and 4 which had the lowest OD value. Group 6 had higher OD value than in Group 5 (P < 0.05). Histological findings of periodontal ligament cell, after being stained with MTT solution were consistent with the in vivo MTT assay results.

In this study, the groups which were frozen with DMSO as a cryoprotectant and the groups with F medium showed the best results.

Citations

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