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- Original Article The comparison of gene expression from human dental pulp cells and periodontal ligament cells
- Hyoun So, Sang-Hyuk Park, Gi-Woon Choi
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2009;34(5):-441.
DOI: https://doi.org/10.5395/JKACD.2009.34.5.430
Published online: September 30, 2009
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- Corresponding Author: Gi-Woon Choi. Department of Conservative Dentistry, Division of Dentistry, Graduate of Kyung Hee University, 1, Hoegi Dong, Dongdaemun Gu, Seoul, Korea, 130-702. Tel: 82-2-958-9336, gwchoi@khu.ac.kr
• Received: August 4, 2009 • Revised: September 3, 2009 • Accepted: September 4, 2009
Copyright © 2009 The Korean Academy of Conservative Dentistry
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Abstract
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The purpose of this study was to characterize functional distinction between human dental pulp cells(PC) and periodontal ligament cells(PDLC) using cDNA microarray assay and to confirm the results of the microarray assay using RT-PCR. 3 genes out of 51 genes which were found to be more expressed(>2 fold) in PC were selected, and 3 genes out of 19 genes which were found to be more expressed(>2 fold) in PDLC were selected for RT-PCR as well.According to this study, the results were as follows:1. From the microarray assay, 51 genes were more expressed (2 fold) from PC than PDLC.2. RT-PCR confirmed that ITGA4 and TGF β2 were more expressed in PC than in PDLC.3. From the microarray assay, 19 genes were more expressed (2 fold) from PDLC than PC.4. RT-PCR confirmed that LUM, WISP1, and MMP1 were more expressed in PDLC than in PC.From the present study, different expression of the genes between the PC and PDLC were characterized to show the genes which play an important role in dentinogenesis were more expressed from PC than PDLC, while the genes which were related with collagen synthesis were more expressed from PDLC than PC.
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REFERENCES
Figure 3
M-A plot
The log ratios(M=log2R/G) are plotted on the y-axis against the log of the geometric mean of the signal intensities (A=log2R×G/2). M, expression ratio; A, signal intensity; R, Red for Cy5; G, Green for Cy3.
Figure 4
Confirmation of differentially expressed genes observed in microarray results. Three genes (ITGA, NEFH, TGF β2) selected from the genes express more than two fold in pulp cells than periodontal ligament cells were analysed by RT-PCR.
Figure 5
Confirmation of differentially expressed genes observed in microarray results. Three genes (MMP1, WISP, LUM) selected from the genes express more than two fold in periodontal ligament cells than pulp cells were analysed by RT-PCR.
Tables & Figures
REFERENCES
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- Gene expression profiling in human dental pulp cells treated with mineral trioxide aggregate
Yong-Beom Kim, Won-Jun Shon, WooCheol Lee, Kee-Yeon Kum, Seung-Ho Baek, Kwang-Shik Bae
Journal of Korean Academy of Conservative Dentistry.2010; 35(3): 152. CrossRef
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XML DownloadThe comparison of gene expression from human dental pulp cells and periodontal ligament cells
Figure 1
Overview of DNA microarray methology
Figure 2
Microarray Scanning Image
Figure 3
M-A plot
The log ratios(M=log2R/G) are plotted on the y-axis against the log of the geometric mean of the signal intensities (A=log2R×G/2). M, expression ratio; A, signal intensity; R, Red for Cy5; G, Green for Cy3.
Figure 4
Confirmation of differentially expressed genes observed in microarray results. Three genes (ITGA, NEFH, TGF β2) selected from the genes express more than two fold in pulp cells than periodontal ligament cells were analysed by RT-PCR.
Figure 5
Confirmation of differentially expressed genes observed in microarray results. Three genes (MMP1, WISP, LUM) selected from the genes express more than two fold in periodontal ligament cells than pulp cells were analysed by RT-PCR.
Figure 1
Figure 2
Figure 3
Figure 4
Figure 5
The comparison of gene expression from human dental pulp cells and periodontal ligament cells
Primers for RT-PCR
The genes expressed more than two fold from the cells cultured with PC than PDLC Ratio = log2 Cy5/Cy3 (Cy5: PDLC, Cy3: PC)
The genes expressed more than two fold from the cells cultured with PDLC than PC Ratio = log2 Cy5/Cy3 (Cy5: PDLC, Cy3: PC)
Table 1
Primers for RT-PCR
Table 2
The genes expressed more than two fold from the cells cultured with PC than PDLC Ratio = log2 Cy5/Cy3 (Cy5: PDLC, Cy3: PC)
Table 3
The genes expressed more than two fold from the cells cultured with PDLC than PC Ratio = log2 Cy5/Cy3 (Cy5: PDLC, Cy3: PC)
