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HOME > Restor Dent Endod > Volume 28(4); 2003 > Article
Original Article Distribution of oral pathogens in infections of endodontic origin
Seung-Yoon Kim, Ho-Young Choi, Sang-Hyuk Park, Gi-Woon Choi
2003;28(4):-313.
DOI: https://doi.org/10.5395/JKACD.2003.28.4.303
Published online: July 31, 2003

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Corresponding author (gwchoi@khu.ac.kr)

Copyright © 2003 Korean Academy of Conservative Dentistry

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  • It has been documented that periodontopathic bacteria are also implicated in endodontic infections. 16S rDNA gene-directed PCR was to examine the prevalence of periodontopathic bacteria including Actinobacillus actinomycetemcomitans (Aa), Prevotella intermedia (Pi), Prevotella nigrescens (Pn), Porphyromonas gingivalis (Pg), Porphyromonas endodontalis (Pe), and Treponema denticola (Td) in the root canals of 36 endodontically infected teeth having apical lesions with or without clinical symptoms like pain, swelling, and fistula.
    1. In 36 infected root canals, most frequently detected bacterial species was Pg (61.1%), followed by Td (52.8%) and Pe (38.9%).

    2. Of 36 infected root canals, Aa was detected in 6 canals (16.7%) of the teeth, all of which showed clinical symptoms.

    3. Of 36 infected root canals, Pi and Pn were found in 4 (13.9%) and 5 (33.3%), respectively. Notably, prevalence of Pn in the symptomatic teeth was 50.0%.

    4. One of black-pigmented anaerobic bacteria (BPB) including Pi, Pn, Pe, and Pg was detected in all of the teeth that showed pain or especially swelling but not fistula. It was, however, found that prevalence of BPB in the asymptomatic teeth or the teeth with fistula was only 40%.

    5. Pe and Pg were detected in the teeth regardless of the presence or absence of symptoms.

    6. Td was detected in the teeth regardless of the presence or absence of symptoms.

    High prevalence of BPB in the symptomatic teeth but low in the asymptomatic teeth suggests that BPB may play an important role in the pathogenesis of periapical lesions.
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Fig. 1
Electrophoresis results of PCR amplification of the control reference DNA of standard bacterial strains. Lanes: M, 100 bp molecular marker; 1, ubiquitous (602 bp); 2, A. actinomycetemcomitans (557 bp); 3, Pr. intermedia (575 bp); 4, Pr. nigrescens (804 bp); P. endodontalis (672 bp); P. gingivalis (404 bp); T. denticola (316 bp).
jkacd-28-303-g001.jpg
Table 1
PCR primer pairs used in this study for detection of putative endodontopathogens
jkacd-28-303-i001.jpg
Table 2
Distribution of bacteria in infection with apical lesions.

Abbreviations: P, Pain; S, Swelling; AA, alveolar abscess; F, fistula; Aa, A. actinomycetemcomitans; Pi, Pr. intermedia; Pn, Pr. nigrescens; Pe, P. endodontalis ; Pg, P. gingivalis ; Td, T. denticola ; a:-denotes no symptom

jkacd-28-303-i002.jpg
Table 3
Distribution of bacteria in fistula*; Samples were obtained through the opening of the fistula, whereas the others from the infected the root canals.
jkacd-28-303-i003.jpg
Table 4
Distribution of bacteria in necrotic root canal sample of apical lesions, having symptoms of pain and/or swelling
jkacd-28-303-i004.jpg
Table 5
Distribution of bacteria in necrotic root canal samples of apical lesions without clinical symptoms
jkacd-28-303-i005.jpg

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      Distribution of oral pathogens in infections of endodontic origin
      J Korean Acad Conserv Dent. 2003;28(4):303-313.   Published online July 31, 2003
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    Distribution of oral pathogens in infections of endodontic origin
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    Fig. 1 Electrophoresis results of PCR amplification of the control reference DNA of standard bacterial strains. Lanes: M, 100 bp molecular marker; 1, ubiquitous (602 bp); 2, A. actinomycetemcomitans (557 bp); 3, Pr. intermedia (575 bp); 4, Pr. nigrescens (804 bp); P. endodontalis (672 bp); P. gingivalis (404 bp); T. denticola (316 bp).
    Distribution of oral pathogens in infections of endodontic origin

    PCR primer pairs used in this study for detection of putative endodontopathogens

    Distribution of bacteria in infection with apical lesions.

    Abbreviations: P, Pain; S, Swelling; AA, alveolar abscess; F, fistula; Aa, A. actinomycetemcomitans; Pi, Pr. intermedia; Pn, Pr. nigrescens; Pe, P. endodontalis ; Pg, P. gingivalis ; Td, T. denticola ; a:-denotes no symptom

    Distribution of bacteria in fistula*; Samples were obtained through the opening of the fistula, whereas the others from the infected the root canals.

    Distribution of bacteria in necrotic root canal sample of apical lesions, having symptoms of pain and/or swelling

    Distribution of bacteria in necrotic root canal samples of apical lesions without clinical symptoms

    Table 1 PCR primer pairs used in this study for detection of putative endodontopathogens

    Table 2 Distribution of bacteria in infection with apical lesions.

    Abbreviations: P, Pain; S, Swelling; AA, alveolar abscess; F, fistula; Aa, A. actinomycetemcomitans; Pi, Pr. intermedia; Pn, Pr. nigrescens; Pe, P. endodontalis ; Pg, P. gingivalis ; Td, T. denticola ; a:-denotes no symptom

    Table 3 Distribution of bacteria in fistula*; Samples were obtained through the opening of the fistula, whereas the others from the infected the root canals.

    Table 4 Distribution of bacteria in necrotic root canal sample of apical lesions, having symptoms of pain and/or swelling

    Table 5 Distribution of bacteria in necrotic root canal samples of apical lesions without clinical symptoms


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