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HOME > Restor Dent Endod > Volume 46(2); 2021 > Article
Research Article Biological assessment of a new ready-to-use hydraulic sealer
Francine Benetti1,2orcid, João Eduardo Gomes-Filho2orcid, India Olinta de Azevedo-Queiroz2orcid, Marina Carminatti2orcid, Letícia Citelli Conti2orcid, Alexandre Henrique dos Reis-Prado1orcid, Sandra Helena Penha de Oliveira3orcid, Edilson Ervolino3orcid, Elói Dezan-Júnior2orcid, Luciano Tavares Angelo Cintra2orcid
Restor Dent Endod 2021;46(2):e21.
DOI: https://doi.org/10.5395/rde.2021.46.e21
Published online: March 24, 2021

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Correspondence to Luciano Tavares Angelo Cintra, PhD. Professor, Department of Preventive and Restorative Dentistry, São Paulo State University (Unesp), School of Dentistry, R: José Bonifácio, 1193. Vila Mendonça. Araçatuba, SP16015-050, Brazil. luciano.cintra@unesp.br
• Received: April 1, 2020   • Revised: May 9, 2020   • Accepted: May 29, 2020

Copyright © 2021. The Korean Academy of Conservative Dentistry

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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  • Objectives
    This study compared the cytotoxicity, biocompatibility, and tenascin immunolabeling of a new ready-to-use hydraulic sealer (Bio-C Sealer) with MTA-Fillapex and white MTA-Angelus.
  • Materials and Methods
    L929 fibroblasts were cultivated and exposed to undiluted and diluted material extracts. Polyethylene tubes with or without (the control) the materials were implanted into the dorsa of rats. At 7 days and 30 days, the rats were euthanized, and the specimens were prepared for analysis; inflammation and immunolabeling were measured, and statistical analysis was performed (p < 0.05).
  • Results
    MTA-Fillapex exhibited greater cytotoxicity than the other materials at all time points (p < 0.05). The undiluted Bio-C Sealer exhibited greater cytocompatibility at 6 and 48 hours than white MTA-Angelus, with higher cell viability than in the control (p < 0.05). White MTA-Angelus displayed higher cell viability than the control at 24 hours, and the one-half dilution displayed similar results at both 6 and 48 hours (p < 0.05). At 7 days and 30 days, the groups exhibited moderate inflammation with thick fibrous capsules and mild inflammation with thin fibrous capsules, respectively (p > 0.05). At 7 days, moderate to strong immunolabeling was observed (p > 0.05). After 30 days, the control and MTA-Fillapex groups exhibited strong immunolabeling, the white MTA-Angelus group exhibited moderate immunolabeling (p > 0.05), and the Bio-C Sealer group exhibited low-to-moderate immunolabeling, differing significantly from the control (p < 0.05).
  • Conclusions
    Bio-C Sealer and white MTA-Angelus exhibited greater cytocompatibility than MTA-Fillapex; all materials displayed adequate biocompatibility and induced tenascin immunolabeling.
  • Keywords: Biocompatibility; Cytotoxicity; Hydraulic sealer; Mineral trioxide aggregate; Tenascin
Mineral trioxide aggregate (MTA) is widely used in the development of calcium silicate materials due to its relative biocompatibility and sealing properties [1]. These materials, known as bioceramics or hydraulic sealers, are highly bioactive, capable of promoting cell differentiation and inducing osteoconductive effects [1,2].
Hydraulic materials are composed of tricalcium and dicalcium silicate, or tricalcium aluminate [3], and can produce hydroxyapatite [4]. This enables a functional integration with dentin [5], favoring the clinical use of hydraulic materials as either restorative materials or sealers for root canal fillings.
MTA-Fillapex (Angelus, Londrina, PR, Brazil) is a hydraulic sealer that has been extensively studied [6,7,8]. This sealer contains MTA particles with dicalcium and tricalcium silicate, resin (salicylate resin and diluted resin) [9], and bismuth oxide as a radiopacifier (Table 1). MTA-Fillapex was developed with the objective of combining the physicochemical properties of a root canal sealer and the bioactivity of MTA [6]. However, despite its satisfactory dimensional changes, radiopacity, and solubility, no consensus has been reached regarding its biocompatibility relative to other sealers [7,8]. For instance, high cytotoxicity of MTA-Fillapex was observed in in vitro studies, owing to its inability to release the ions necessary for apatite formation [7,8]. Studies have attributed these results to the presence of resin in the composition of MTA-Fillapex [10,11]. In contrast, an in vivo analysis has demonstrated that this material is biocompatible and capable of inducing biomineralization in rat connective tissue [9].
Table 1

Composition of the materials and manufacturers’ instructions for use

Material Composition Instructions for use
MTA Fillapex • Salicylate resin, diluting resin, natural resin, bismuth trioxide, nanoparticulated silica, MTA • Dual syringe: Press plunger to extrude material directly onto a glass slab, onto a mixing pad, or into the tooth canal. The cement should be used immediately after mixing.
• Tubes: Mix equal-volume units (1:1). Mix for 30 seconds to attain a homogeneous consistency.
Bio-C Sealer • Calcium silicates, calcium aluminate, calcium oxide, zirconium oxide, iron oxide, silicon dioxide and dispersing agent • Ready for use.
White MTA-Angelus • Powder: tricalcium silicate, dicalcium silicate, tricalcium aluminate, calcium oxide, bismuth oxide • For 30 seconds, mix the content of 1 sachet of MTA-Angelus (or 1 spoon of MTA-Angelus) with 1 drop of distilled water. The final mixture should be homogeneous and have a consistency similar to wet sand.
• Liquid: distilled water
MTA, mineral trioxide aggregate.
In the search for an ideal sealer material, several bioceramic materials have been introduced to the market [12]. To this end, a new hydraulic sealer was developed containing silicates, aluminate, calcium oxide, ferric oxide, zirconium, and silicon dioxide in addition to other dispersing agents (Table 1). This sealer, known as Bio-C Sealer (Angelus), is available in a syringe, with no need for spatulation.
Studies using rat subcutaneous tissue have highlighted important results regarding the effects of different endodontic materials on markers of inflammation and biomineralization [13,14,15,16]. For example, a comparative analysis of collagen maturation or fibroblast growth factor in rat subcutaneous tissue revealed that materials with different compositions could influence tissue response [17]. Furthermore, studies have shown that MTA could induce the expression of tenascin [18,19], a non-collagenous glycoprotein of the extracellular matrix that plays important roles in migration, adhesion, cell proliferation [18,20], and mineralization [18,21]. Therefore, in addition to indicating a tissue healing process, the expression of this protein may induce biomineralization. However, these phenomena have not been evaluated for endodontic sealers.
Despite its ease of clinical handling and the considerable attention that Bio-C Sealer has attracted in endodontics, its manufacturers do not provide information regarding the sealer's filler material, pigments, and diluents. For this reason, an initial analysis of its components is challenging. Thus, the aim of the present study was to evaluate the cytotoxicity, biocompatibility, and potential of Bio-C Sealer for the immunolabeling of tenascin compared to MTA-Fillapex and white MTA-Angelus.
In vitro study: cell viability assay
The Bio-C Sealer used in this study was acquired in ready-to-use form, while the MTA-Fillapex and white MTA-Angelus materials were mixed according to the manufacturers' recommendations (Table 1). The material discs were prepared using sterile and cylindrical polyethylene tubes (diameter and height, 5 and 3 mm, respectively) [13,22] and incubated at 37°C and 100% relative humidity for 6 hours. These were then sterilized using ultraviolet irradiation for 1 hour, and material extracts were prepared according to International Organization for Standardization (ISO) specifications [13,22,23]. Three different dilutions (undiluted, one-half, and one-fourth) of the material extracts were used.
For the viability assay, fibroblasts from the L929 cell line were cultured under standard cell culture conditions using Dulbecco's modified Eagle medium (DMEM; Sigma-Aldrich, St. Louis, MO, USA) supplemented with fetal bovine serum (10%, Sigma-Aldrich), streptomycin, and penicillin (temperature, 37°C; humidity, 100%; air, 95%; and CO2, 5%). Cells were subsequently seeded and incubated in 96-well plates (Sigma-Aldrich) at 104 cells/well for 24 hours under standard conditions. Next, serial extract dilutions were prepared using either the culture or DMEM without material extracts (the control). After 6, 24, and 48 hours, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) (Sigma-Aldrich) assay [13,22] was performed to determine cell viability. Each condition was analyzed in triplicate.
In vivo study

1. Biocompatibility analysis

Sixteen 2-month-old male Wistar rats (each weighing approximately 280 g) were used in the biocompatibility assay. Previous studies were used to determine the sample size that should be used [13,22]. The animals were housed in a controlled environment (22ºC–24ºC and a light cycle of 12 hours light/dark), with water and food provided ad libitum. All experimental procedures were approved by the ethics committee of the local institution (CEUA 937-2017) and were performed in accordance with the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health (Bethesda, MD, USA).
A total of 48 polyethylene tubes (Abbott Laboratories, São Paulo, SP, Brazil) filled with Bio-C Sealer, MTA-Fillapex, or white MTA-Angelus, as well as 16 empty tubes (used as the control), were randomly and immediately implanted into the rat subcutaneous tissue. Each rat received 4 tubes: 3 material-filled tubes and 1 empty tube. For this purpose, the rats were anesthetized, their dorsa were shaved, and a 2.0-cm incision was made in a head-to-tail orientation with a no. 15 blade; the skin was reflected to create 2 pockets on the right and left sides, after which the randomly selected tubes were implanted [13,22].
After 7 and 30 days, the rats (n = 8) were euthanized with an overdose of an anesthetic solution. The tubes, along with the surrounding tissue, were removed and fixed in a neutral formalin solution. Then, the specimens were processed and embedded into paraffin. The blocks were oriented parallel to the long axis of the tubes, and longitudinal serial sections of 5-µm thickness were obtained from the central areas of the implants. The histological sections were stained using hematoxylin and eosin.
The inflammatory response in the tissues that were in contact with the material in the tube insertion region was evaluated as follows [13,22]: 1) no/few inflammatory cells, no inflammation; 2) < 25 cells, mild inflammation; 3) 25–125 cells, moderate inflammation; and 4) ≥ 126 cells, severe inflammation. Fibrous capsules < 150 µm were considered thin, while those ≥ 150 µm were considered thick. The specimens were examined by a single calibrated operator in a blinded manner using light microscopy (DM 4000 B; Leica Microsystems, Wetzlar, Germany).

2. Immunohistochemical analysis

Immunohistochemical analysis was performed using an indirect immunoperoxidase technique [24]. The histological sections were deparaffinized using xylene and hydrated in ethanol. Antigen retrieval was performed using a citrate buffer solution (Antigen Retrieval Buffer; Spring Bioscience, Pleasanton, CA, USA) in a pressurized chamber (Decloaking Chamber™; Biocare Medical, Concord, CA, USA). The sections were then immersed in 3% H2O2 solution (for 1 hour and 20 minutes) and in 1% bovine serum albumin (for 12 hours) to block endogenous peroxidase activity and non-specific sites, respectively. Next, the sections were incubated with the primary antibody anti-tenascin (rabbit; Sigma-Aldrich) for 24 hours, after which they were incubated with biotinylated secondary antibody (1 hour and 30 minutes) and streptavidin-horseradish peroxidase conjugate (1 hour and 30 minutes) (Universal Dako labeled streptavidin-biotin kit; Dako Laboratories, Santa Clara, CA, USA). The reaction was developed using the chromogen 3,3′-diaminobenzidine tetrahydrochloride (DAB Chromogen kit; Dako Laboratories) and counterstained with hematoxylin. The negative controls consisted of specimens that underwent the previously described procedures without treatment with the primary antibody.
Analyses were performed by a single calibrated and blinded operator using light microscopy (×400 magnification; DM4000 B). The immunolabeling of tenascin was defined as the presence of a brownish color in the cytoplasm of the cells and extracellular matrix. A semi-quantitative analysis revealed the numbers of immunolabeled cells and immunolabeling intensity of the extracellular matrix, as follows [24]: 1) no immunolabeling; 2) mild immunolabeling; 3) moderate immunolabeling; 4) strong immunolabeling; and 5) very strong immunolabeling.
Statistical analysis
The data regarding cytotoxicity were statistically analyzed using 2-way analysis of variance, followed by the Bonferroni correction. The Kruskal-Wallis and Dunn tests were used to analyze biocompatibility and immunohistochemical data, and p values of < 0.05 were considered to indicate statistical significance.
In vitro study: cell viability assay
Data regarding cell viability are shown in Figure 1. MTA-Fillapex decreased the cell viability (undiluted, one-half, and one-fourth dilutions) at all time points compared to the control group (p < 0.05). In contrast, Bio-C Sealer was associated with increased cell viability relative to the control at most time points (p < 0.05). At 24 hours, all white MTA-Angelus extracts increased the cell viability compared to the control (p < 0.05); similar results were obtained at 6 hours and 48 hours for the one-half dilution (p < 0.05).
Figure 1

Cell viability for MTA-Fillapex, Bio-C Sealer, and white MTA-Angelus.

A-DThe same uppercase letters indicate a lack of statistically significant differences among the materials at the same period and in the same dilution. a-cThe same lowercase letters indicate a lack of statistically significant differences among extract dilutions of the same material at the same time point.
rde-46-e21-g001.jpg
At the same dilutions of the material, MTA-Fillapex exhibited greater cytotoxicity than Bio-C Sealer and white MTA-Angelus at all time points (p < 0.05). The undiluted white MTA-Angelus displayed lower cell viability than Bio-C Sealer (p < 0.05), except at 24 hours. Regarding the one-half and one-fourth dilutions of white MTA-Angelus, the overall outcome was similar to that observed with Bio-C Sealer at most time points (p > 0.05).
Comparing the different dilutions of the same material, we observed a greater decrease in cell viability with more concentrated extracts of MTA-Fillapex (p < 0.05). Lower cell viability was also observed for the undiluted Bio-C Sealer compared to its one-half dilution at 6 hours and 48 hours and its one-fourth dilution at 48 hours (p < 0.05). In addition, the one-half dilution of white MTA-Angelus exhibited higher cell viability at 6 hours and 48 hours than the undiluted extract; similar findings were observed when the one-half dilution was compared to the one-fourth dilution at 6 hours and 24 hours (p < 0.05).
In vivo study

1. Biocompatibility analysis

Images of tissue responses are shown in Figure 2A-H and M-T, and the histological analysis is summarized in Table 2. After 7 days, moderate infiltration of polymorphonuclear cells, macrophages, and multinucleated giant cells was observed in the thick fibrous capsules in all groups (p > 0.05).
Figure 2

Representative images of the tissue response in terms of inflammatory infiltration and immunolabeling of tenascin. (A-L) Under the highest magnification, the white arrowheads indicate inflammatory cells, the yellow arrows indicate blood vessels, and asterisks indicate the fibrous capsule. The blue arrowheads indicate immunolabeling in the cytoplasm of the cells and in the extracellular matrix. At 7 days: (A, E) control, (B, F) MTA-Fillapex, (C, G) Bio-C Sealer, and (D, H) white MTA-Angelus displaying mild inflammatory cell infiltration and a thick fibrous capsule; (I) the control group with strong immunolabeling and (J) MTA-Fillapex, (K) Bio-C Sealer, and (L) the white MTA-Angelus groups with moderate immunolabeling. (M-X) At 30 days: (M, Q) the control, (N, R) MTA-Fillapex, (O, S) Bio-C Sealer, and (P, T) white MTA-Angelus, with mild inflammatory infiltration and a thin fibrous capsule; (U) control and (V) MTA-Fillapex groups with strong immunolabeling, (W) Bio-C Sealer with low immunolabeling, and (X) white MTA-Angelus with moderate immunolabeling. (A-D, M-P) The histological sections were stained using hematoxylin and eosin staining (×100 magnification). (E-H, Q-T) The histological sections were stained using hematoxylin and eosin staining (×400 magnification). (I-L, U-X) Results of the immunohistochemical analysis with regard to tenascin (×400 magnification).

rde-46-e21-g002.jpg
Table 2

Scores for inflammation and tenascin expression, as well as thickness of the fibrous capsule

Time/p-value Group Scores for inflammation Median Scores for tenascin Median Fibrous capsule thickness n
1 2 3 4 1 2 3 4 5
7 days ControlAa 0 1 4 3 3 0 2 2 4 0 4 Thick 8
*p = 0.521 MTA-FillapexAa 0 2 4 2 3 0 0 4 2 2 3 Thick
†p = 0.553 Bio-C SealerAa 0 3 4 1 3 0 1 4 3 0 3 Thick
White MTA-AngelusAa 0 2 5 1 3 0 2 4 1 1 3 Thick
30 days ControlAa 2 4 2 0 2 0 0 3 5 0 4 Thin 8
*p = 0.082 MTA-FillapexAab 0 6 2 0 2 0 1 3 4 0 4 Thin
†p = 0.017 Bio-C SealerAb 2 6 0 0 2 0 4 4 0 0 2.5 Thin
White MTA-AngelusAab 0 4 4 0 2.5 0 2 4 2 0 3 Thin
AThe same uppercase letters indicate a lack of a statistically significant difference among the groups with regard to inflammation. a,bThe same lowercase letters indicate a lack of a statistically significant difference among the groups with regard to the immunolabeling of tenascin (p > 0.05).
*p value obtained in the statistical analysis of inflammation, in each analysis period; †p value obtained in the statistical analysis of tenascin immunolabeling, in each analysis period.
After 30 days, most specimens of the groups exhibited mild inflammation with the presence of polymorphonuclear cells and a small number of lymphocytes. Some specimens from the control and Bio-C Sealer groups displayed no inflammation, whereas some MTA-Fillapex specimens displayed moderate inflammation. However, no significant differences were present among the groups (p > 0.05), and the fibrous capsules were thin in this period in all specimens.

2. Immunohistochemical analysis

The images of immunolabeled tenascin are depicted in Figure 2I-L and U-X, and the data are summarized in Table 2. The immunolabeling was observed in the cytoplasm of fibroblastic cells and principally in the extracellular matrix. After 7 days, most specimens in the control group displayed strong immunolabeling, whereas those in the other groups exhibited moderate immunolabeling. However, no significant differences were present among the groups (p > 0.05).
After 30 days, the control and MTA-Fillapex specimens displayed strong immunolabeling, whereas the white MTA-Angelus specimens exhibited moderate immunolabeling. However, the specimens in the Bio-C Sealer group exhibited low-to-moderate immunolabeling, with a significant difference between the control and Bio-C Sealer groups (p < 0.05).
The clinical use of hydraulic sealers has become increasingly common. The present study involved the analysis of the cell viability and biocompatibility of MTA-Fillapex, as well as those of a new formulation of a calcium silicate sealer, the Bio-C Sealer. White MTA-Angelus was used as a reference due to its known cytocompatibility and biocompatibility. In this study, MTA-Fillapex showed similar cytotoxicity to that displayed by the control group and the other materials assessed. In general, Bio-C Sealer and white MTA-Angelus exhibited excellent cytocompatibility. No significant difference was present between the materials in the histological analysis, and all of the materials were considered to be biocompatible. Nevertheless, compared with the control group, the Bio-C Sealer group displayed lower tenascin immunolabeling after 30 days.
The cytotoxicity of MTA-Fillapex has been previously evaluated using different cell types, including primary human osteoblasts [25], cells of the human periodontal ligament [8], NIH/3T3 cells [1], and human dental pulp stem cells [26]. In the present study, L929 fibroblasts were used. The undiluted and different dilutions of the material extracts were used in a similar fashion to previous studies (ISO 10993-5:2009) [13,22,23]. After the insertion of the material in the tissue, the extracellular fluids gradually eliminate leachable compounds, and their local concentrations progressively decrease as represented by the distinct dilutions [13,27].
In a previous study, the cytotoxicity of MTA-Fillapex was detected in the form of a defective organization of the F-actin cytoskeleton. This structure controls cell surface motion, which is important for cell proliferation and differentiation [28]. However, the formation of a slower-growing mineralized matrix with functional activity was observed [11]. According to the authors of that study, cytotoxicity occurred most prominently during the adjustment period; however, recovery of the cells and mineralization of the matrix were observed later. In contrast, in the present study, cytotoxicity was observed after adjustment of the material, as the extracts were obtained after this stage.
Moreover, when in contact with osteoblast cells in a previous study, MTA-Fillapex exhibited cytotoxicity in a fresh as well as in a set state [25], which corroborates our in vitro results, but contradicts our in vivo results, in which the material was used while still fresh. We must consider that the negative results obtained with cell studies can be influenced by the conditions of in vitro studies. In this case, the analysis was performed in cellular monolayers, whereas in in vivo studies, it was conducted on tissues with a continuous flow of fluid, which may favor the biocompatibility of the material [11]. In addition, a 3-dimensional system can be more appropriated than the traditional 2-dimensional system for in vitro analysis [29], which is a limitation of this study.
For the in vivo analysis, we implanted tubes filled with the evaluated materials (as well as empty tubes) into the subcutaneous tissue of rats, in the method recommended by ISO 10993 [30]. This experimental condition simulated the reactions of the periapical tissues when in contact with a still-fresh sealer. A previous study reported that MTA-Fillapex resulted in reduced irritation of the subcutaneous tissue of rats after 60 days, with the fibrous capsule containing some fibroblasts and a few inflammatory cells [31]. These results are consistent with those of the present study, in which we observed some inflammatory cells, most notably after 7 days. Consequently, the fibrous capsule was observed to be thin after 30 days, in contrast to the thick capsule observed at the 7-day mark.
Thus, MTA-Fillapex has a limited toxic effect on living tissues, while other endodontic sealers are capable of releasing toxic components for a prolonged period. Similarly, a zinc oxide-eugenol–based sealer has been shown to provoke strong irritation in the living tissues over time [13,31], while MTA-Fillapex was associated with lower immunoexpression of interleukin-6 in an in vivo analysis relative to other sealers, such as AH Plus [31].
The presence of resins has been associated with the cytotoxicity of MTA-Fillapex [10,11], which has been attributed to the release of arsenic [10]. Arsenic is a heavy metal that is capable of inducing genotoxicity [32]. Furthermore, aluminum was found to be present in the circulation of rats after the use of MTA-Fillapex, and this increased the levels of oxidative stress in the erythrocytes and livers of these animals [33,34]. However, aluminum release was observed with MTA-Angelus [34], even though this material generally has high cytocompatibility. Based on these results, the ability of a material to release aluminum cannot be considered to be related to its level of cytotoxicity, and further studies are needed to reach a firm conclusion.
In addition, in the present study, MTA-Fillapex allowed the expression of tenascin, which is associated with tissue repair as well as cellular motility [21]. Studies have indicated that tenascin is necessary for mineralization [18,20]. Although the present study was conducted in connective tissue with an absence of cells suitable for biomineralization, such as odontoblasts or osteoblasts, the immunolabeling of tenascin indicated not only a repair process, but also the induction of biomineralization [18,21].
In alignment with the results of this study, previous studies have reported good cell viability with MTA-Angelus in L929 fibroblasts [22] as well as in other cell types, such as dental pulp stem cells [26], human dermal fibroblasts [35], and odontoblast-like cells [36]. Moreover, the tissue biocompatibility of white MTA-Angelus has been previously demonstrated [22]. However, no study has evaluated the biological properties of the new hydraulic Bio-C Sealer, which makes comparison difficult. Regarding the physicochemical properties of this sealer, a recent study revealed a short setting time and satisfactory flow in compliance with the ISO 6876 standard; in contrast to the AH Plus sealer, which has been shown to be associated with a relatively low pH, it exhibits a low volumetric change and alkalinization capacity due to the release of OH− and Ca2+ ions [37].
In addition to calcium silicate, the Bio-C Sealer contains aluminate and calcium oxide, along with zirconium oxide, iron oxide, and silicon dioxide. Calcium aluminate supports the acquisition of osteogenic cell phenotypes in vitro [38], and calcium aluminate materials exhibit good biocompatibility in the subcutaneous tissue of rats [39], which can explain our results. Moreover, calcium oxide reacts with tissue fluids, stimulating the deposition of hard tissue through the release of calcium ions [40].
The radiopacity of the Bio-C Sealer material was considered adequate and met the American National Standards Institute/American Dental Association criteria [37]. Unlike MTA-Fillapex, the Bio-C Sealer includes zirconium oxide as a radiopacifier, and this substance is associated with the proliferation of fibroblasts and the acceleration of the regression of inflammatory reactions [41]. Other studies reported the cytotoxic effects of bismuth oxide in different cell types [42,43]. This validates the results found in the present study and could be a reason for studying a new formulation of MTA-Fillapex, in which calcium tungstate is used instead of bismuth oxide [44].
In contrast to the abovementioned studies, the present study demonstrated that the immunolabeling of tenascin increased after 30 days in all groups except the Bio-C Sealer group. Although tenascin immunolabeling has already been shown to generally decrease over time, especially after mineralization [18], the results of the present study suggested relatively less intense induction of biomineralization associated with the Bio-C Sealer. Therefore, further studies are needed to gain a better understanding of the properties of this sealer, such as the transplantation of tricalcium phosphate/hydroxyapatite–containing extract-treated cells.
Bio-C Sealer is a new formulation hydraulic sealer with potential applications in root canal fillings. Based on the findings of this study, this new material exhibited higher cell viability at most evaluation time points and dilutions relative to MTA-Fillapex and white MTA-Angelus. In addition to being biocompatible, Bio-C Sealer could serve as an alternative for endodontic sealers. However, additional investigations are required to verify its physical, chemical, and bioactive properties prior to its indication for clinical use.
Endodontic sealers are materials that remain in close contact with living tissues. Thus, the development of new, biologically acceptable materials with more appropriate physiochemical properties is a priority. In this study, the Bio-C Sealer material displayed cytocompatibility, biocompatibility, and tenascin expression similar to those exhibited by white MTA-Angelus.

Funding: This research received support from the Conselho Nacional de Desenvolvimento Científico e Tecnológico – CNPq (305969/2015-3), São Paulo, SP, Brazil.

Conflict of Interest: No potential conflict of interest relevant to this article was reported.

Author Contributions:

  • Conceptualization: Cintra LTA, Benetti F, Gomes-Filho JE.

  • Data curation: Azevedo-Queiroz IO, Carminatti M, Conti LC, Reis-Prado AH.

  • Formal analysis: Benetti F, Azevedo-Queiroz IO, Carminatti M, Conti LC, Reis-Prado AH, Ervolino E.

  • Investigation: Benetti F, Azevedo-Queiroz IO, Carminatti M, Conti LC, Reis-Prado AH.

  • Methodology: Cintra LTA, Benetti F, Gomes-Filho JE, Azevedo-Queiroz IO.

  • Project administration: Cintra LTA, Oliveira SHP, Ervolino E, Dezan-Júnior E.

  • Resources: Cintra LTA, Oliveira SHP, Ervolino E, Dezan-Junior E.

  • Software: Cintra LTA, Dezan-Junior E.

  • Supervision: Cintra LTA, Gomes-Filho JE, Dezan-Júnior E.

  • Validation: Cintra LTA, Gomes-Filho JE, Oliveira SHP, Ervolino E, Dezan-Júnior E.

  • Writing - original draft: Benetti F, Carminatti M, Conti LC, Reis-Prado AH.

  • Writing - review & editing: Gomes-Filho JE, Cintra LTA.

  • 1. Vouzara T, Dimosiari G, Koulaouzidou EA, Economides N. Cytotoxicity of a new calcium silicate endodontic sealer. J Endod 2018;44:849-852.ArticlePubMed
  • 2. Shie MY, Chang HC, Ding SJ. Effects of altering the Si/Ca molar ratio of a calcium silicate cement on in vitro cell attachment. Int Endod J 2012;45:337-345.ArticlePubMed
  • 3. Saghiri MA, Orangi J, Asatourian A, Gutmann JL, Garcia-Godoy F, Lotfi M, Sheibani N. Calcium silicate-based cements and functional impacts of various constituents. Dent Mater J 2017;36:8-18.ArticlePubMed
  • 4. Han L, Okiji T. Uptake of calcium and silicon released from calcium silicate-based endodontic materials into root canal dentine. Int Endod J 2011;44:1081-1087.ArticlePubMed
  • 5. Reyes-Carmona JF, Felippe MS, Felippe WT. The biomineralization ability of mineral trioxide aggregate and Portland cement on dentin enhances the push-out strength. J Endod 2010;36:286-291.ArticlePubMed
  • 6. Vitti RP, Prati C, Silva EJ, Sinhoreti MA, Zanchi CH, de Souza e Silva MG, Ogliari FA, Piva E, Gandolfi MG. Physical properties of MTA Fillapex sealer. J Endod 2013;39:915-918.ArticlePubMed
  • 7. Zhou HM, Du TF, Shen Y, Wang ZJ, Zheng YF, Haapasalo M. In vitro cytotoxicity of calcium silicate-containing endodontic sealers. J Endod 2015;41:56-61.ArticlePubMed
  • 8. Collado-González M, Tomás-Catalá CJ, Oñate-Sánchez RE, Moraleda JM, Rodríguez-Lozano FJ. Cytotoxicity of GuttaFlow Bioseal, GuttaFlow2, Mta Fillapex, and Ah Plus on human periodontal ligament stem cells. J Endod 2017;43:816-822.ArticlePubMed
  • 9. Gomes-Filho JE, Watanabe S, Lodi CS, Cintra LT, Nery MJ, Filho JA, Dezan E Jr, Bernabé PF. Rat tissue reaction to MTA Fillapex® . Dent Traumatol 2012;28:452-456.ArticlePubMed
  • 10. Salles LP, Gomes-Cornélio AL, Guimarães FC, Herrera BS, Bao SN, Rossa-Junior C, Guerreiro-Tanomaru JM, Tanomaru-Filho M. Mineral trioxide aggregate-based endodontic sealer stimulates hydroxyapatite nucleation in human osteoblast-like cell culture. J Endod 2012;38:971-976.ArticlePubMed
  • 11. Costa F, Sousa Gomes P, Fernandes MH. Osteogenic and angiogenic response to calcium silicate-based endodontic sealers. J Endod 2016;42:113-119.ArticlePubMed
  • 12. Grossman LI. Endodontic practice. 10th ed. Philadelphia, PA: Lea & Febiger; 1982.
  • 13. Cintra LTA, Benetti F, de Azevedo Queiroz ÍO, Ferreira LL, Massunari L, Bueno CRE, de Oliveira SHP, Gomes-Filho JE. Evaluation of the cytotoxicity and biocompatibility of new resin epoxy-based endodontic sealer containing calcium hydroxide. J Endod 2017;43:2088-2092.ArticlePubMed
  • 14. Andrade AS, Silva GF, Camilleri J, Cerri ES, Guerreiro-Tanomaru JM, Cerri PS, Tanomaru-Filho M. Tissue response and immunoexpression of interleukin 6 promoted by tricalcium silicate-based repair materials after subcutaneous implantation in rats. J Endod 2018;44:458-463.ArticlePubMed
  • 15. Cosme-Silva L, Benetti F, Dal-Fabbro R, Gomes Filho JE, Sakai VT, Cintra LTA, Alvarez N, Ervolino E, Viola NV. Biocompatibility and biomineralization ability of Bio-C Pulpecto. A histological and immunohistochemical study. Int J Paediatr Dent 2019;29:352-360.ArticlePubMedPDF
  • 16. Cosme-Silva L, Gomes-Filho JE, Benetti F, Dal-Fabbro R, Sakai VT, Cintra LTA, Ervolino E, Viola NV. Biocompatibility and immunohistochemical evaluation of a new calcium silicate-based cement, Bio-C Pulpo. Int Endod J 2019;52:689-700.ArticlePubMedPDF
  • 17. da Fonseca TS, Silva GF, Guerreiro-Tanomaru JM, Sasso-Cerri E, Tanomaru-Filho M, Cerri PS. Mast cells and immunoexpression of FGF-1 and Ki-67 in rat subcutaneous tissue following the implantation of Biodentine and MTA Angelus. Int Endod J 2019;52:54-67.ArticlePubMedPDF
  • 18. Zarrabi MH, Javidi M, Jafarian AH, Joushan B. Immunohistochemical expression of fibronectin and tenascin in human tooth pulp capped with mineral trioxide aggregate and a novel endodontic cement. J Endod 2011;37:1613-1618.ArticlePubMed
  • 19. Moradi S, Saghravanian N, Moushekhian S, Fatemi S, Forghani M. Immunohistochemical evaluation of fibronectin and tenascin following direct pulp capping with mineral trioxide aggregate, platelet-rich plasma and propolis in dogs’ teeth. Iran Endod J 2015;10:188-192.PubMedPMC
  • 20. Thesleff I, Mackie E, Vainio S, Chiquet-Ehrismann R. Changes in the distribution of tenascin during tooth development. Development 1987;101:289-296.ArticlePubMedPDF
  • 21. Piva E, Tarquínio SB, Demarco FF, Silva AF, de Araújo VC. Immunohistochemical expression of fibronectin and tenascin after direct pulp capping with calcium hydroxide. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;102:e66-e71.ArticlePubMed
  • 22. Cintra LTA, Benetti F, de Azevedo Queiroz ÍO, de Araújo Lopes JM, Penha de Oliveira SH, Sivieri Araújo G, Gomes-Filho JE. Cytotoxicity, biocompatibility, and biomineralization of the new high-plasticity MTA material. J Endod 2017;43:774-778.ArticlePubMed
  • 23. International Organization for Standardization (ISO). ISO 10993-5: Biological evaluation of medical devices - Part 5: Tests for in vitro cytotoxicity. Geneva: ISO; 2009.
  • 24. Benetti F, Briso ALF, Carminatti M, de Araújo Lopes JM, Barbosa JG, Ervolino E, Gomes-Filho JE, Cintra LTA. The presence of osteocalcin, osteopontin and reactive oxygen species-positive cells in pulp tissue after dental bleaching. Int Endod J 2019;52:665-675.ArticlePubMedPDF
  • 25. Jung S, Sielker S, Hanisch MR, Libricht V, Schäfer E, Dammaschke T. Cytotoxic effects of four different root canal sealers on human osteoblasts. PLoS One 2018;13:e0194467.ArticlePubMedPMC
  • 26. Victoria-Escandell A, Ibañez-Cabellos JS, de Cutanda SB, Berenguer-Pascual E, Beltrán-García J, García-López E, Pallardó FV, García-Giménez JL, Pallarés-Sabater A, Zarzosa-López I, Monterde M. Cellular responses in human dental pulp stem cells treated with three endodontic materials. Stem Cells Int 2017;2017:8920356.ArticlePubMedPMCPDF
  • 27. Benetti F, Queiroz ÍOA, Cosme-Silva L, Conti LC, Oliveira SHP, Cintra LTA. Cytotoxicity, biocompatibility and biomineralization of a new ready-for-use bioceramic repair material. Braz Dent J 2019;30:325-332.ArticlePubMed
  • 28. Titushkin I, Cho M. Modulation of cellular mechanics during osteogenic differentiation of human mesenchymal stem cells. Biophys J 2007;93:3693-3702.ArticlePubMedPMC
  • 29. Silva EJ, Carvalho NK, Ronconi CT, De-Deus G, Zuolo ML, Zaia AA. Cytotoxicity profile of endodontic sealers provided by 3D cell culture experimental model. Braz Dent J 2016;27:652-656.ArticlePubMed
  • 30. International Organization for Standardization (ISO). ISO 10993-6: Biological evaluation of medical devices - Part 6: Testes for local effects after implantation. Geneva: ISO; 2016.
  • 31. Saraiva JA, da Fonseca TS, da Silva GF, Sasso-Cerri E, Guerreiro-Tanomaru JM, Tanomaru-Filho M, Cerri PS. Reduced interleukin-6 immunoexpression and birefringent collagen formation indicate that MTA Plus and MTA Fillapex are biocompatible. Biomed Mater 2018;13:035002.ArticlePubMedPDF
  • 32. Monteiro Bramante C, Demarchi AC, de Moraes IG, Bernadineli N, Garcia RB, Spångberg LS, Duarte MA. Presence of arsenic in different types of MTA and white and gray Portland cement. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2008;106:909-913.ArticlePubMed
  • 33. Demirkaya K, Can Demirdöğen B, Öncel Torun Z, Erdem O, Çetinkaya S, Akay C. In vivo evaluation of the effects of hydraulic calcium silicate dental cements on plasma and liver aluminium levels in rats. Eur J Oral Sci 2016;124:75-81.ArticlePubMed
  • 34. Demirkaya K, Demirdöğen BC, Torun ZO, Erdem O, Çırak E, Tunca YM. Brain aluminium accumulation and oxidative stress in the presence of calcium silicate dental cements. Hum Exp Toxicol 2017;36:1071-1080.ArticlePubMedPDF
  • 35. Hirschman WR, Wheater MA, Bringas JS, Hoen MM. Cytotoxicity comparison of three current direct pulp-capping agents with a new bioceramic root repair putty. J Endod 2012;38:385-388.ArticlePubMed
  • 36. Lessa FC, Aranha AM, Hebling J, Costa CA. Cytotoxic effects of White-MTA and MTA-Bio cements on odontoblast-like cells (MDPC-23). Braz Dent J 2010;21:24-31.ArticlePubMed
  • 37. Zordan-Bronzel CL, Esteves Torres FF, Tanomaru-Filho M, Chávez-Andrade GM, Bosso-Martelo R, Guerreiro-Tanomaru JM. Evaluation of physicochemical properties of a new calcium silicate-based sealer, Bio-C Sealer. J Endod 2019;45:1248-1252.ArticlePubMed
  • 38. Castro-Raucci LMS, Teixeira LN, Oliveira IR, Raucci-Neto W, Jacobovitz M, Rosa AL, de Oliveira PT. Osteogenic cell response to calcium aluminate-based cement. Int Endod J 2017;50:771-779.ArticlePubMedPDF
  • 39. Garcia Lda F, Huck C, Menezes de Oliveira L, de Souza PP, de Souza Costa CA. Biocompatibility of new calcium aluminate cement: tissue reaction and expression of inflammatory mediators and cytokines. J Endod 2014;40:2024-2029.ArticlePubMed
  • 40. Bernabé PF, Gomes-Filho JE, Rocha WC, Nery MJ, Otoboni-Filho JA, Dezan-Júnior E. Histological evaluation of MTA as a root-end filling material. Int Endod J 2007;40:758-765.ArticlePubMed
  • 41. Silva GF, Guerreiro-Tanomaru JM, da Fonseca TS, Bernardi MIB, Sasso-Cerri E, Tanomaru-Filho M, Cerri PS. Zirconium oxide and niobium oxide used as radiopacifiers in a calcium silicate-based material stimulate fibroblast proliferation and collagen formation. Int Endod J 2017;50(Supplement 2):e95-e108.ArticlePubMedPDF
  • 42. Chiang TY, Ding SJ. Comparative physicochemical and biocompatible properties of radiopaque dicalcium silicate cement and mineral trioxide aggregate. J Endod 2010;36:1683-1687.ArticlePubMed
  • 43. Min KS, Chang HS, Bae JM, Park SH, Hong CU, Kim EC. The induction of heme oxygenase-1 modulates bismuth oxide-induced cytotoxicity in human dental pulp cells. J Endod 2007;33:1342-1346.ArticlePubMed
  • 44. Kebudi Benezra M, Schembri Wismayer P, Camilleri J. Interfacial characteristics and cytocompatibility of hydraulic sealer cements. J Endod 2018;44:1007-1017.ArticlePubMed

Tables & Figures

REFERENCES

    Citations

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      Journal of Conservative Dentistry.2022; 25(2): 145.     CrossRef

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      Biological assessment of a new ready-to-use hydraulic sealer
      Restor Dent Endod. 2021;46(2):e21  Published online March 24, 2021
      DOI: https://doi.org/10.5395/rde.2021.46.e21
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    Biological assessment of a new ready-to-use hydraulic sealer
    Image Image
    Figure 1 Cell viability for MTA-Fillapex, Bio-C Sealer, and white MTA-Angelus.A-DThe same uppercase letters indicate a lack of statistically significant differences among the materials at the same period and in the same dilution. a-cThe same lowercase letters indicate a lack of statistically significant differences among extract dilutions of the same material at the same time point.
    Figure 2 Representative images of the tissue response in terms of inflammatory infiltration and immunolabeling of tenascin. (A-L) Under the highest magnification, the white arrowheads indicate inflammatory cells, the yellow arrows indicate blood vessels, and asterisks indicate the fibrous capsule. The blue arrowheads indicate immunolabeling in the cytoplasm of the cells and in the extracellular matrix. At 7 days: (A, E) control, (B, F) MTA-Fillapex, (C, G) Bio-C Sealer, and (D, H) white MTA-Angelus displaying mild inflammatory cell infiltration and a thick fibrous capsule; (I) the control group with strong immunolabeling and (J) MTA-Fillapex, (K) Bio-C Sealer, and (L) the white MTA-Angelus groups with moderate immunolabeling. (M-X) At 30 days: (M, Q) the control, (N, R) MTA-Fillapex, (O, S) Bio-C Sealer, and (P, T) white MTA-Angelus, with mild inflammatory infiltration and a thin fibrous capsule; (U) control and (V) MTA-Fillapex groups with strong immunolabeling, (W) Bio-C Sealer with low immunolabeling, and (X) white MTA-Angelus with moderate immunolabeling. (A-D, M-P) The histological sections were stained using hematoxylin and eosin staining (×100 magnification). (E-H, Q-T) The histological sections were stained using hematoxylin and eosin staining (×400 magnification). (I-L, U-X) Results of the immunohistochemical analysis with regard to tenascin (×400 magnification).

    Figure 1

    Figure 2

    Biological assessment of a new ready-to-use hydraulic sealer

    Composition of the materials and manufacturers’ instructions for use

    MaterialCompositionInstructions for use
    MTA Fillapex• Salicylate resin, diluting resin, natural resin, bismuth trioxide, nanoparticulated silica, MTA• Dual syringe: Press plunger to extrude material directly onto a glass slab, onto a mixing pad, or into the tooth canal. The cement should be used immediately after mixing.
    • Tubes: Mix equal-volume units (1:1). Mix for 30 seconds to attain a homogeneous consistency.
    Bio-C Sealer• Calcium silicates, calcium aluminate, calcium oxide, zirconium oxide, iron oxide, silicon dioxide and dispersing agent• Ready for use.
    White MTA-Angelus• Powder: tricalcium silicate, dicalcium silicate, tricalcium aluminate, calcium oxide, bismuth oxide• For 30 seconds, mix the content of 1 sachet of MTA-Angelus (or 1 spoon of MTA-Angelus) with 1 drop of distilled water. The final mixture should be homogeneous and have a consistency similar to wet sand.
    • Liquid: distilled water

    MTA, mineral trioxide aggregate.

    Scores for inflammation and tenascin expression, as well as thickness of the fibrous capsule

    Time/p-valueGroupScores for inflammationMedianScores for tenascinMedianFibrous capsule thicknessn
    123412345
    7 daysControlAa01433022404Thick8
    *p = 0.521MTA-FillapexAa02423004223Thick
    †p = 0.553Bio-C SealerAa03413014303Thick
    White MTA-AngelusAa02513024113Thick
    30 daysControlAa24202003504Thin8
    *p = 0.082MTA-FillapexAab06202013404Thin
    †p = 0.017Bio-C SealerAb26002044002.5Thin
    White MTA-AngelusAab04402.5024203Thin

    AThe same uppercase letters indicate a lack of a statistically significant difference among the groups with regard to inflammation. a,bThe same lowercase letters indicate a lack of a statistically significant difference among the groups with regard to the immunolabeling of tenascin (p > 0.05).

    *p value obtained in the statistical analysis of inflammation, in each analysis period; †p value obtained in the statistical analysis of tenascin immunolabeling, in each analysis period.
    Table 1 Composition of the materials and manufacturers’ instructions for use

    MTA, mineral trioxide aggregate.

    Table 2 Scores for inflammation and tenascin expression, as well as thickness of the fibrous capsule

    AThe same uppercase letters indicate a lack of a statistically significant difference among the groups with regard to inflammation. a,bThe same lowercase letters indicate a lack of a statistically significant difference among the groups with regard to the immunolabeling of tenascin (p > 0.05).

    *p value obtained in the statistical analysis of inflammation, in each analysis period; †p value obtained in the statistical analysis of tenascin immunolabeling, in each analysis period.

    Table 1

    Table 2

    Restor Dent Endod : Restorative Dentistry & Endodontics
    © The Korean Academy of Conservative Dentistry.
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