Department of Conservative Dentistry, Division of Dentistry, Graduate School of Kyung Hee University, Korea.
Copyright © 2003 Korean Academy of Conservative Dentistry
a; Cells grown up to their optical density of 0.6 at 540 nm were further incubated with various reagents alone or in combination and then intracellular nucleotide release from the cells was determined by measuring the absorbance at 260 nm.
a; Cells grown up to their optical density of 0.6 at 540 nm were further incubated with various reagents alone or in combination and then intracellular nucleotide release from the cells was determined by measuring the absorbance at 260 nm.
a; The experiment was performed as described in Table 4 except that the amount of antibiotics added was doubled.
a; Antibiotics at lower concentrations with or without Calgon (0.1~1.0%) were added to the culture and incubated as described in Table 3. The optical density of growing E. faecalis cells was determined at 540 nm.
a; Calgon at the concentrations of 0.1~1.0% was added to an inoculum of E. faecalis in BHI at the very beginning of the culture and incubated for 12 h anaerobically. Change in the growth of the bacterial cells was determined by measuring the optical density at 540 nm.
Abbreviation; AP, ampicillin; CFX, cefotaxime; EM, erythromycin; GM, gentamicin; KM, kanamycin; PN-G, pencillin-G; TC, tetracycline.
a; Cells grown up to their optical density of 0.6 at 540 nm were further incubated with various reagents alone or in combination and then intracellular nucleotide release from the cells was determined by measuring the absorbance at 260 nm.
a; Cells grown up to their optical density of 0.6 at 540 nm were further incubated with various reagents alone or in combination and then intracellular nucleotide release from the cells was determined by measuring the absorbance at 260 nm.
a; The experiment was performed as described in Table 4 except that the amount of antibiotics added was doubled.
a; Antibiotics at lower concentrations with or without Calgon (0.1~1.0%) were added to the culture and incubated as described in Table 3. The optical density of growing E. faecalis cells was determined at 540 nm.
a; Calgon at the concentrations of 0.1~1.0% was added to an inoculum of E. faecalis in BHI at the very beginning of the culture and incubated for 12 h anaerobically. Change in the growth of the bacterial cells was determined by measuring the optical density at 540 nm.
Abbreviation; AP, ampicillin; CFX, cefotaxime; EM, erythromycin; GM, gentamicin; KM, kanamycin; PN-G, pencillin-G; TC, tetracycline.
a; Antibiotics at lower concentrations with or without Calgon (0.1~1.0%) were added to the culture and incubated as described in Table 3. The optical density of growing E. faecalis cells was determined at 540 nm.
a; Calgon at the concentrations of 0.1~1.0% was added to an inoculum of E. faecalis in BHI at the very beginning of the culture and incubated for 12 h anaerobically. Change in the growth of the bacterial cells was determined by measuring the optical density at 540 nm.
Abbreviation; AP, ampicillin; CFX, cefotaxime; EM, erythromycin; GM, gentamicin; KM, kanamycin; PN-G, pencillin-G; TC, tetracycline.
a; Calgon at the concentrations of 0.1~1.0% was added to an inoculum of E. faecalis in BHI at the very beginning of the culture and incubated for 12 h anaerobically. Change in the growth of the bacterial cells was determined by measuring the optical density at 540 nm.
Leakage of intracellular nucleotide from P. gingivalis A7A1-28 in the presence of polyP75a
a; Cells grown up to their optical density of 0.6 at 540 nm were further incubated with various reagents alone or in combination and then intracellular nucleotide release from the cells was determined by measuring the absorbance at 260 nm.
Leakage of intracellular nucleotide from P. gingivalis W50 in the presence of polyP75a
a; Cells grown up to their optical density of 0.6 at 540 nm were further incubated with various reagents alone or in combination and then intracellular nucleotide release from the cells was determined by measuring the absorbance at 260 nm.
Effect of polyP (Calgon) on the growth of E. faecalis ATCC 29212a
a; Calgon at the concentrations of 0.1~1.0% was added to an inoculum of E. faecalis in BHI at the very beginning of the culture and incubated for 12 h anaerobically. Change in the growth of the bacterial cells was determined by measuring the optical density at 540 nm.
Permeabilizing effect of polyP on sensitivity of E. faecalis to various antibiotics at lower concentrations as measured the bacterial optical density at 540 nma
a; Antibiotics at lower concentrations with or without Calgon (0.1~1.0%) were added to the culture and incubated as described in
Abbreviation; AP, ampicillin; CFX, cefotaxime; EM, erythromycin; GM, gentamicin; KM, kanamycin; PN-G, pencillin-G; TC, tetracycline.
Permeabilizing effect of polyP on sensitivity of E. faecalis to various antibiotics at higher concentrations as measured the bacterial optical density at 540 nma
a; The experiment was performed as described in
a; Cells grown up to their optical density of 0.6 at 540 nm were further incubated with various reagents alone or in combination and then intracellular nucleotide release from the cells was determined by measuring the absorbance at 260 nm.
a; Cells grown up to their optical density of 0.6 at 540 nm were further incubated with various reagents alone or in combination and then intracellular nucleotide release from the cells was determined by measuring the absorbance at 260 nm.
a; Calgon at the concentrations of 0.1~1.0% was added to an inoculum of E. faecalis in BHI at the very beginning of the culture and incubated for 12 h anaerobically. Change in the growth of the bacterial cells was determined by measuring the optical density at 540 nm.
a; Antibiotics at lower concentrations with or without Calgon (0.1~1.0%) were added to the culture and incubated as described in
Abbreviation; AP, ampicillin; CFX, cefotaxime; EM, erythromycin; GM, gentamicin; KM, kanamycin; PN-G, pencillin-G; TC, tetracycline.
a; The experiment was performed as described in